Recombinant construct P{Yes-3.BS}

General Information
Symbol	P{Yes-3.BS}	FlyBase ID	FBtp0020624
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zhang and Tower, 2004)
Uses	CV term Qualifiers & info Reference characterization ACE3 (Zhang and Tower, 2004) Ori66Dbeta (Zhang and Tower, 2004) Ori66Dβ (Zhang and Tower, 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	ACE3³²⁰
Reference(s)	(Zhang and Tower, 2004)
Molecular data	320bp fragment. (Lu et al., 2001)
Phenotypic class
Phenotype manifest in
Other information

Allele	Cp15^tZa
Reference(s)	(Zhang and Tower, 2004)
Molecular data	Construct: Genomic fragment containing the complete Cp15 transcript. (Zhang and Tower, 2004)
Phenotypic class
Phenotype manifest in
Other information

Allele	Cp18^ch1
Reference(s)	(Zhang and Tower, 2004)
Molecular data	Construct: 3.8kb SalI genomic fragment containing the Cp15 and Cp18 transcripts. (Rubin and Spradling, 1982)
Phenotypic class
Phenotype manifest in
Other information

Allele	gypsy\su(Hw)BR⁴³⁰
Reference(s)	(Zhang and Tower, 2004)
Molecular data	Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon. (Kuhn et al., 2003) Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014). (Roseman et al., 1995) Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014. (Roseman et al., 1993) Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites. (Patton et al., 1992)
Phenotypic class
Phenotype manifest in
Other information

Allele	Ori66Dβ⁸⁸⁴
Reference(s)	(Zhang and Tower, 2004)
Molecular data	884 bp fragment of Ori66Dβ. (Lu et al., 2001)
Phenotypic class
Phenotype manifest in
Other information

Allele	y^+t*
Reference(s)	(Zhang and Tower, 2004)
Molecular data
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	construct_comment: P{Yes-3.BS} contains (5\' to 3\'): gypsy\su(Hw)BR⁴³⁰ fused to the the y gene, fused to a genomic fragment containing ACE3 (+ some 5\' flanking sequence) , Cp1, Ori66Dβ and Cp15, fused at the 3\' end to an inverted gypsy\su(Hw)BR⁴³⁰. (Zhang and Tower, 2004) P{Yes-3.BS} contains (5\' to 3\'): gypsy\su(Hw)BR⁴³⁰ fused to the the y gene, fused to a genomic fragment containing ACE3 (+ some 5\' flanking sequence) , Cp1, Ori66Dβ and Cp15, fused at the 3\' end to an inverted gypsy\su(Hw)BR⁴³⁰. (Zhang and Tower, 2004)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{Yes-3.BS} (Zhang and Tower, 2004, FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Zhang and Tower, 2004, Development 131(9): 2089--2099 Sequence requirements for function of the Drosophila chorion gene locus ACE3 replicator and ori- origin elements. [FBrf0174575]