Recombinant construct P{UAS-Adar.3.4}

General Information
Symbol	P{UAS-Adar.3.4}	FlyBase ID	FBtp0021070
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Keegan et al., 2005)
Uses	CV term Qualifiers & info Reference binary system (targeted) Adar (Keegan et al., 2005) characterization Adar (Keegan et al., 2005)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Keegan et al., 2005) selectable marker (Keegan et al., 2005)
Component Alleles
Allele	Adar^3.4.Scer\UAS
Reference(s)	(Keegan et al., 2005, Keegan et al., 2011, Bhogal et al., 2011)
Molecular data	Construct: Scer\UAS sequences, fused to a truncated hsp70 promoter, drives expression of cDNA encoding the Adar 3/4 isoform, tagged with T:Zzzz\FLAG and T:Zzzz\His6. (Keegan et al., 2005)
Phenotypic class	partially lethal - majority die \| male, with Adar^1F4, Scer\GAL4^Act5C.PI (Keegan et al., 2005) viable, with Scer\GAL4^Mef2.PR (Keegan et al., 2005)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Keegan et al., 2005)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Adar 3/4 un (Keegan et al., 2011) P{UAS-Adar.3.4} UAS-Adar (Bhogal et al., 2011) UAS-Adar 3a S/G (Keegan et al., 2005) UAS-dAdar (Keegan et al., 2011)
Secondary FlyBase IDs

References ( 4 )
Research paper	Bhogal et al., 2011, Nat. Neurosci. 14(12): 1517--1524 Modulation of dADAR-dependent RNA editing by the Drosophila fragile X mental retardation protein. [FBrf0216747] Keegan et al., 2011, Nucleic Acids Res. 39(16): 7249--7262 Functional conservation in human and Drosophila of Metazoan ADAR2 involved in RNA editing: loss of ADAR1 in insects. [FBrf0215032] Keegan et al., 2005, EMBO J. 24(12): 2183--2193 Tuning of RNA editing by ADAR is required in Drosophila. [FBrf0187510]
Supplementary material	Horiuchi et al., 2005, Curr. Biol. 15(23): Supplemental data. [FBrf0191753]