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General Information
Symbol
P{gcid-EGFP-cid}
FlyBase ID
FBtp0021159
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
EGFP-Cid, gcid-EGFP-cid
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Marker(s)
Component allele cidEGFP
Product class / Tool use(s)
Regulatory region(s)
Description

P{gcid-EGFP-cid} is composed of three distinct fragments. First, a 772 bp cid PCR fragment encompassing 412 bp upstream of the cid translational initiation codon and 354 bp of the coding region up to codon 118. Second, a PCR fragment encompassing the cid region coding for amino acids 119 through 225 as well as 513 bp of the flanking 3' genomic region. A third fragment encompassing the EGFP coding region, and flanked by three glycine residues at both sides to serve as flexible linkers, is inserted between the cid codons specifying amino acids 118 and 119. Thus, EGFP is inserted within the N-terminal tail of the cid protein.

A genomic fragment of cid is expressed from its own promoter and has EGFP fused within the fragment 11 amino acids before the start of the histone fold.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
selectable
marker
Component Alleles
Allele
Molecular data

A genomic fragment of cid is expressed from its own promoter and has EGFP fused within the fragment 11 amino acids before the start of the histone fold.

P{gcid-EGFP-cid} is composed of three distinct fragments. First, a 772 bp cid PCR fragment encompassing 412 bp upstream of the cid translational initiation codon and 354 bp of the coding region up to codon 118. Second, a PCR fragment encompassing the cid region coding for amino acids 119 through 225 as well as 513 bp of the flanking 3' genomic region. A third fragment encompassing the EGFP coding region, and flanked by three glycine residues at both sides to serve as flexible linkers, is inserted between the cid codons specifying amino acids 118 and 119. Thus, EGFP is inserted within the N-terminal tail of the cid protein.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (13)
References (17)