Recombinant construct P{UAS-zip.GFP.DN}

General Information
Symbol	P{UAS-zip.GFP.DN}	FlyBase ID	FBtp0021310
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Franke et al., 2005)
Uses	CV term Qualifiers & info Reference binary system (targeted) zip (Franke et al., 2005) characterization zip (Franke et al., 2005) reporter construct vital \| zip (Franke et al., 2005)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Franke et al., 2005) molecular tag (Franke et al., 2005) selectable marker (Franke et al., 2005)
Component Alleles
Allele	w^+mC
Reference(s)	(Franke et al., 2005)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	zip^{DN.Scer\UAS.T:Avic\GFP}
Reference(s)	(Franke et al., 2005, Tang et al., 2011, Monier et al., 2010, Hatan et al., 2011, Olguín et al., 2011, Franke et al., 2010)
Molecular data	Construct: Scer\UAS sequences drive expression of a zip fragment (amino acid residues 775-1972) tagged with T:Avic\GFP at the N terminus. (Franke et al., 2005)
Phenotypic class	lethal, with Scer\GAL4^en-e16E (Franke et al., 2010) lethal, with Scer\GAL4^en-e16E, zip¹ (Franke et al., 2010) planar polarity defective \| heat sensitive, with Scer\GAL4^pnr-MD237, Scer\GAL80^ts.αTub84B (Olguín et al., 2011) short lived \| calorie restriction conditional, with Scer\GAL4^Cg.PA (Tang et al., 2011) visible, with Scer\GAL4^ap-md544 (Franke et al., 2010) visible, with Scer\GAL4^en-e16E (Franke et al., 2010)
Phenotype manifest in	embryonic dorsal epidermis, with Scer\GAL4^en-e16E, zip¹ (Franke et al., 2005) macrochaeta, with Scer\GAL4^ap-md544 (Franke et al., 2010) mesothoracic tergum \| heat sensitive, with Scer\GAL4^pnr-MD237, Scer\GAL80^ts.αTub84B (Olguín et al., 2011) microchaeta, with Scer\GAL4^ap-md544 (Franke et al., 2010) trichome, with Scer\GAL4^ap-md544 (Franke et al., 2010) trichome \| supernumerary, with Scer\GAL4^ap-md544 (Franke et al., 2010) wing blade, with Scer\GAL4^ap-md544 (Franke et al., 2010) wing blade \| posterior compartment, with Scer\GAL4^en-e16E (Franke et al., 2010) wing hair, with Scer\GAL4^en-e16E (Franke et al., 2010) wing hair \| supernumerary, with Scer\GAL4^en-e16E (Franke et al., 2010)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	DN-zip (Olguín et al., 2011) GFP-DN-zip (Franke et al., 2005) P{UAS-zip.GFP.DN} pUAS-GFP-zip/MyoII-Neck-Rod (Franke et al., 2010) UAS>GFP-DN-MHC (Monier et al., 2010) UAS-ZIP-DN (Tang et al., 2011) UAS-Zipper^DN-GFP (Hatan et al., 2011)
Secondary FlyBase IDs

References ( 7 )
Research paper	Hatan et al., 2011, J. Cell Biol. 192(2): 307--319 The Drosophila blood brain barrier is maintained by GPCR-dependent dynamic actin structures. [FBrf0212807] Olguín et al., 2011, Curr. Biol. 21(3): 236--242 Intertissue mechanical stress affects frizzled-mediated planar cell polarity in the Drosophila notum epidermis. [FBrf0212947] Tang et al., 2011, EMBO J. 30(4): 636--651 Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy. [FBrf0213047] Franke et al., 2010, Dev. Biol. 345(2): 117--132 Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis. [FBrf0211576] Monier et al., 2010, Nat. Cell Biol. 12(1): 60--65 An actomyosin-based barrier inhibits cell mixing at compartmental boundaries in Drosophila embryos. [FBrf0209576] Franke et al., 2005, Curr. Biol. 15(24): 2208--2221 Nonmuscle myosin II generates forces that transmit tension and drive contraction in multiple tissues during dorsal closure. [FBrf0190012]
Supplementary material	Horiuchi et al., 2005, Curr. Biol. 15(23): Supplemental data. [FBrf0191753]