Recombinant construct P{UAS-EcR.A.W650A}

General Information
Symbol	P{UAS-EcR.A.W650A}	FlyBase ID	FBtp0021360
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Brown et al., 2006)
Uses	CV term Qualifiers & info Reference binary system (targeted) EcR (Brown et al., 2006) characterization EcR (Brown et al., 2006)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Brown et al., 2006)
Component Alleles
Allele	EcR^{A-ΔC655.W650A.Scer\UAS}
Reference(s)	(Brown et al., 2006, Chawla and Sokol, 2012, Brown and Truman, 2009, Wang et al., 2011)
Molecular data	Construct: Amino acid replacement: W650A. Scer\UAS regulatory sequences drive expression of a mutated EcR A isoform, with the above amino acid replacement, that is truncated after residue 655. (Brown et al., 2006)
Phenotypic class	male fertile, with Scer\GAL4^nos.PG (Wang et al., 2011) neuroanatomy defective (Brown and Truman, 2009) neuroanatomy defective, with Scer\GAL4^Fmrf.PS (Brown et al., 2006)
Phenotype manifest in	leg \| adult stage \| somatic clone, with Scer\GAL4^elav-C155 (Brown and Truman, 2009) mesothoracic ventral neurosecretory neuron & axon, with Scer\GAL4^Fmrf.PS (Brown et al., 2006) metathoracic ventral thoracic neurosecretory neuron & axon, with Scer\GAL4^Fmrf.PS (Brown et al., 2006) motor neuron \| adult stage \| somatic clone, with Scer\GAL4^elav-C155 (Brown and Truman, 2009) neurohemal organ, with Scer\GAL4^Fmrf.PS (Brown et al., 2006) prothoracic ventral neurosecretory neuron & axon, with Scer\GAL4^Fmrf.PS (Brown et al., 2006)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	9451 w^*; P{UAS-EcR.A.W650A}TP5
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	EcR-DN (Chawla and Sokol, 2012) EcR DN (Chawla and Sokol, 2012) P{UAS-EcR.A.W650A} UAS-EcRA(DN) (Wang et al., 2011) UAS-EcR-A^W650A (Brown et al., 2006, Brown and Truman, 2009) UAS-EcR A DN (Chawla and Sokol, 2012) UAS-EcRA-W650A (Chawla and Sokol, 2012) UAS-EcR-DN (Chawla and Sokol, 2012)
Secondary FlyBase IDs

References ( 5 )
Research paper	Chawla and Sokol, 2012, Development 139(10): 1788--1797 Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function. [FBrf0218077] Wang et al., 2011, Dev. Biol. 351(1): 146--155 The cholesterol trafficking protein NPC1 is required for Drosophila spermatogenesis. [FBrf0212972] Brown and Truman, 2009, Development 136(19): 3247--3256 Fine-tuning of secondary arbor development: the effects of the ecdysone receptor on the adult neuronal lineages of the Drosophila thoracic CNS. [FBrf0209119] Brown et al., 2006, Development 133(2): 275--285 Use of time-lapse imaging and dominant negative receptors to dissect the steroid receptor control of neuronal remodeling in Drosophila. [FBrf0190279]
Supplementary material	Horiuchi et al., 2005, Curr. Biol. 15(23): Supplemental data. [FBrf0191753]