Recombinant construct P{UAS-br.Z3}

General Information
Symbol	P{UAS-br.Z3}	FlyBase ID	FBtp0021399
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zhou et al., 2009)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Zhou et al., 2009)
Component Alleles
Allele	br^Z3.Scer\UAS
Reference(s)	(Schubiger et al., 2005, Costantino et al., 2008, Mirth et al., 2009, Zhou et al., 2009, Zhou et al., 2004, Xiang et al., 2010)
Molecular data	Scer\UAS regulatory sequences drive expression of cDNA clone "dm797" which encodes the br Z3 isoform. (Zhou et al., 2009) Construct: Scer\UAS regulatory sequences drive expression of br-Z3 isoform coding sequences. (Schubiger et al., 2005)
Phenotypic class	lethal, with Scer\GAL4^Feb211 (Zhou et al., 2004) lethal, with Scer\GAL4^P0206 (Zhou et al., 2004) lethal \| embryonic stage, with Scer\GAL4^elav-C155 (Zhou et al., 2009) lethal \| first instar larval stage, with Scer\GAL4^elav-C155 (Zhou et al., 2009) lethal \| pharate adult stage, with Scer\GAL4^Feb204 (Zhou et al., 2004) lethal \| pupal stage P7, with Scer\GAL4^Feb204 (Zhou et al., 2004) locomotor behavior defective \| first instar larval stage, with Scer\GAL4^elav-C155 (Zhou et al., 2009) locomotor behavior defective \| second instar larval stage, with Scer\GAL4^elav-C155 (Zhou et al., 2009) neuroanatomy defective \| adult stage, with Scer\GAL4^P2.4.Pdf (Zhou et al., 2009)
Phenotype manifest in	LNv neuron, with Scer\GAL4^P2.4.Pdf (Zhou et al., 2009) prothoracic gland, with Scer\GAL4^P0206 (Zhou et al., 2004)
Other information

Allele	w^+mC
Reference(s)	(Zhou et al., 2009)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Br-Z3 (Schubiger et al., 2005) P{UAS-br.Z3} UAS-BRC-Z3 (Costantino et al., 2008) UAS br Z3 (Mirth et al., 2009) UAS BR-Z3 (Zhou et al., 2004) UAS-Z3 (Xiang et al., 2010)
Secondary FlyBase IDs

References ( 6 )
Research paper	Xiang et al., 2010, Dev. Biol. 344(2): 800--808 br regulates the expression of the ecdysone biosynthesis gene npc1. [FBrf0211381] Mirth et al., 2009, Development 136(14): 2345--2353 The Ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs. [FBrf0208188] Zhou et al., 2009, Neural Dev. 4(1): 39 Temporal patterns of broad isoform expression during the development of neuronal lineages in Drosophila. [FBrf0209341] Costantino et al., 2008, PLoS Genet. 4(6): e1000102 A novel ecdysone receptor mediates steroid-regulated developmental events during the mid-third instar of Drosophila. [FBrf0205257] Schubiger et al., 2005, Development 132(23): 5239--5248 Ligand-dependent de-repression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing. [FBrf0190319] Zhou et al., 2004, J. Exp. Biol. 207(7): 1151--1161 Overexpression of broad: a new insight into its role in the Drosophila prothoracic gland cells. [FBrf0174866]