Recombinant construct M{3xP3-RFP.attP}

General Information
Symbol	M{3xP3-RFP.attP}	FlyBase ID	FBtp0021844
Feature type	transgenic_transposon	Created / Updated	2007-06-14/2007-06-14

Size		Expression data
Location-dependent role		Associated insertions	61 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon Dmau\mariner (Bischof et al., 2006)
Uses	CV term Qualifiers & info Reference target element recombination-mediated genomic insertion \| phiC31\attP (Bischof et al., 2006)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent EF362407
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Bischof et al., 2006)
Component Alleles
Disc\RFP^3xP3.PB
Reference(s)	(Bischof et al., 2006)
Molecular data	An artificial "3xP3" promoter drives expression of Disc\RFP. The construct has a αTub84B 3' UTR. (Bischof et al., 2006)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)	M{vas-int.Dm} (Bischof and Basler, 2007.11.7) M{UAST-lacZ.B} M{vas-int.B} M{vas-int.NLS} M{nos-int.B} M{nos-int.NLS} M{loxP.attP} (Bischof et al., 2007, Bischof et al., 2007, Bischof et al., 2007, Bischof et al., 2007, Bischof et al., 2007, Bischof et al., 2007)
Comments
	M{3xP3-RFP.attP} contains the Disc\RFP[3xP3.PB] marker flanked by P1\loxP sites with a phiC31\attP site at the 3' end and is designed to be a landing site for a phiC31-based integration system. (Bischof et al., 2006)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	M{3xP3-RFP.attP} (FlyBase, 1996-) M{3xP3-RFPattP} (Bischof et al., 2007) pM3xP3-RFPattP (Bischof et al., 2006) pM{3xP3-RFPattP} (Bischof et al., 2006) pmar(3)-3xP3-RFP-attP-mar(5) (Bischof et al., 2006)
Secondary FlyBase IDs

References ( 6 )
Research paper	Bischof et al., 2007, Proc. Natl. Acad. Sci. USA 104(9): 3312--3317 An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases. [FBrf0192974]
Supplementary material	Bischof et al., 2006, Proc. Natl. Acad. Sci. USA 104(9): SI Text. [FBrf0198668] Bischof et al., 2006, Proc. Natl. Acad. Sci. USA 104(9): SI Table 4. [FBrf0195884] Bischof et al., 2006, Proc. Natl. Acad. Sci. USA 104(9): SI Figure 5. [FBrf0198670]
Personal communication to FlyBase	Bischof and Basler, 2007.11.7, Information on M{vas-int.Dm} stocks. Information on M{vas-int.Dm} stocks. [FBrf0200341]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]