Recombinant construct P{Act5C(-FRT)GAL4.Switch.PR}

General Information
Symbol	P{Act5C(-FRT)GAL4.Switch.PR}	FlyBase ID	FBtp0021868
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	9 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Rogulja and Irvine, 2005)
Uses	CV term Qualifiers & info Reference binary system (regulatory) Act5C (Rogulja and Irvine, 2005)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Rogulja and Irvine, 2005)
Component Alleles
Allele	Scer\GAL4^{Act5C.Switch.PR}
Reference(s)	(Rogulja and Irvine, 2005, Zhang et al., 2010, Ren et al., 2009, Ford et al., 2007, Zid et al., 2009, Baumgartner et al., 2010, Kallio et al., 2010, Wang et al., 2010, Wigby et al., 2011, Shen et al., 2009, Alic et al., 2011, Paik et al., 2012)
Molecular data
Phenotypic class	aging defective, with CoVb^GD6659 (Zid et al., 2009) aging defective, with l(3)neo18^GD3876 (Zid et al., 2009) immune response defective \| RU486 conditional, with Atg5^{dsRNA.Scer\UAS} (Ren et al., 2009) immune response defective \| RU486 conditional, with Atg7^{dsRNA.Scer\UAS} (Ren et al., 2009) immune response defective \| RU486 conditional, with Atg12^{dsRNA.Scer\UAS} (Ren et al., 2009) increased cell death \| somatic clone \| RU486 conditional, with kibra^Scer\UAS.cBa (Baumgartner et al., 2010) increased cell number \| cell non-autonomous \| somatic clone \| third instar larval stage, with tkv^{DE-Q199D.Scer\UAS.T:Ivir\HA1} (Rogulja and Irvine, 2005) increased cell number \| cell non-autonomous \| somatic clone \| third instar larval stage, with tkv^{Q253D.Scer\UAS.cNc} (Rogulja and Irvine, 2005) increased cell number \| somatic clone \| third instar larval stage, with tkv^{DE-Q199D.Scer\UAS.T:Ivir\HA1} (Rogulja and Irvine, 2005) increased cell number \| somatic clone \| third instar larval stage, with tkv^{Q253D.Scer\UAS.cNc} (Rogulja and Irvine, 2005) lethal \| larval stage \| RU486 conditional, with Ras85D^V12.Scer\UAS (Shen et al., 2009) lethal \| larval stage \| RU486 conditional, with wg^{Scer\UAS.cHa.T:Ivir\HA1} (Shen et al., 2009) long lived \| female \| RU486 conditional, with p53^259H.GUS (Shen et al., 2009) long lived \| female limited \| RU486 conditional, with ImpL2^Scer\UAS.cHa (Alic et al., 2011) long lived \| RU486 conditional, with fabp^Scer\UAS.cLa (Lee et al., 2012) mating defective \| RU486 conditional, with InR^{K1409A.Scer\UAS} (Wigby et al., 2011) mitotic cell cycle defective \| cell non-autonomous \| somatic clone \| third instar larval stage, with tkv^{DE-Q199D.Scer\UAS.T:Ivir\HA1} (Rogulja and Irvine, 2005) mitotic cell cycle defective \| cell non-autonomous \| somatic clone \| third instar larval stage, with tkv^{Q253D.Scer\UAS.cNc} (Rogulja and Irvine, 2005) mitotic cell cycle defective \| somatic clone \| third instar larval stage, with tkv^{DE-Q199D.Scer\UAS.T:Ivir\HA1} (Rogulja and Irvine, 2005) mitotic cell cycle defective \| somatic clone \| third instar larval stage, with tkv^{Q253D.Scer\UAS.cNc} (Rogulja and Irvine, 2005) mitotic cell cycle defective \| third instar larval stage, with tkv^{Q253D.Scer\UAS.cNc} (Rogulja and Irvine, 2005) short lived, with ImpL2^s.Scer\UAS (Paik et al., 2012) short lived \| male limited, with MRG15^GD11902 (Zhang et al., 2010) short lived \| RU486 conditional, with BacA\p35^Scer\UAS.cHa (Shen et al., 2009) short lived \| RU486 conditional, with Ras85D^V12.Scer\UAS (Shen et al., 2009) short lived \| RU486 conditional, with wg^{Scer\UAS.cHa.T:Ivir\HA1} (Shen et al., 2009) stress response defective, with CoVb^GD6659 (Zid et al., 2009) stress response defective, with l(3)neo18^GD3876 (Zid et al., 2009) stress response defective \| female limited \| RU486 conditional, with ImpL2^Scer\UAS.cHa (Alic et al., 2011) stress response defective \| RU486 conditional, with et^GD6535 (Kallio et al., 2010) stress response defective \| RU486 conditional, with et^KK105015 (Kallio et al., 2010) stress response defective \| RU486 conditional, with Stat92E^GD4492 (Kallio et al., 2010)
Phenotype manifest in	wing disc \| cell non-autonomous \| somatic clone \| third instar larval stage, with tkv^{DE-Q199D.Scer\UAS.T:Ivir\HA1} (Rogulja and Irvine, 2005) wing disc \| cell non-autonomous \| somatic clone \| third instar larval stage, with tkv^{Q253D.Scer\UAS.cNc} (Rogulja and Irvine, 2005) wing disc \| somatic clone \| RU486 conditional, with kibra^Scer\UAS.cBa (Baumgartner et al., 2010) wing disc \| somatic clone \| third instar larval stage, with tkv^{DE-Q199D.Scer\UAS.T:Ivir\HA1} (Rogulja and Irvine, 2005) wing disc \| somatic clone \| third instar larval stage, with tkv^{Q253D.Scer\UAS.cNc} (Rogulja and Irvine, 2005) wing disc \| third instar larval stage, with tkv^{Q253D.Scer\UAS.cNc} (Rogulja and Irvine, 2005)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	9431 P{hsFLP}22, y¹ w^*; P{UAS-GFP.S65T}Myo31DF^T2; P{Act5C(-FRT)GAL4.Switch.PR}3/TM6B, Tb¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Act5C (actin5C) P '^Switch' GAL4 (Zid et al., 2009) Act-Gal4:PR (Baumgartner et al., 2010) ActGS (Alic et al., 2011) Act-GS-255A (Shen et al., 2009) Act-GS-255B (Zhang et al., 2010, Shen et al., 2009) act-GS-Gal4 (Paik et al., 2012) actin>Gal4:PR (Rogulja and Irvine, 2005) Actin GeneSwitch (Alic et al., 2011) GeneSwitch-GAL4 (Kallio et al., 2010) GS-255A (Wigby et al., 2011) GS255A (Wigby et al., 2011) GS-Act-255B (Ren et al., 2009) GS-Actin255A (Shen et al., 2009) GS-Actin255-B (Shen et al., 2009) GS-GAL4 (Kallio et al., 2010) P{Act5C(-FRT)GAL4.Switch.PR} (FlyBase, 1992-, Ford et al., 2007)
Secondary FlyBase IDs

References ( 12 )
Research paper	Paik et al., 2012, Mech. Ageing Dev. 133(5): 234--245 Misexpression screen delineates novel genes controlling Drosophila lifespan. [FBrf0218389] Alic et al., 2011, Aging Cell 10(1): 137--147 Lifespan extension by increased expression of the Drosophila homologue of the IGFBP7 tumour suppressor. [FBrf0212768] Wigby et al., 2011, Proc. Biol. Sci. 278(1704): 424--431 Insulin signalling regulates remating in female Drosophila. [FBrf0212666] Kallio et al., 2010, FASEB J. 24(11): 4467--4479 Eye transformer is a negative regulator of Drosophila JAK/STAT signaling. [FBrf0212202] Wang et al., 2010, J. Mol. Cell. Biol. 2(3): 128--138 20-hydroxyecdysone reduces insect food consumption resulting in fat body lipolysis during molting and pupation. [FBrf0210866] Zhang et al., 2010, Exp. Gerontol. 45(11): 825--833 Conditional inactivation of MRG15 gene function limits survival during larval and adult stages of Drosophila melanogaster. [FBrf0212068] Ren et al., 2009, Exp. Gerontol. 44(3): 228--235 Conditional inhibition of autophagy genes in adult Drosophila impairs immunity without compromising longevity. [FBrf0206988] Shen et al., 2009, Aging 1(2): 191--211 A screen of apoptosis and senescence regulatory genes for life span effects when over-expressed in Drosophila. [FBrf0209964] Zid et al., 2009, Cell 139(1): 149--160 4E-BP extends lifespan upon dietary restriction by enhancing mitochondrial activity in Drosophila. [FBrf0209084] Ford et al., 2007, Exp. Gerontol. 42(6): 483--497 Alteration of Drosophila life span using conditional, tissue-specific expression of transgenes triggered by doxycyline or RU486/Mifepristone. [FBrf0200753] Rogulja and Irvine, 2005, Cell 123(3): 449--461 Regulation of cell proliferation by a morphogen gradient. [FBrf0189921]
Supplementary material	Baumgartner et al., 2010, Dev. Cell 18(2): Supplemental Information. [FBrf0211373]