Recombinant construct P{Car20y}

General Information
Symbol	P{Car20y}	FlyBase ID	FBtp0022033
Feature type	transgenic_transposon

Size	31.313	Expression data
Associated insertions	13 available
Molecular map
Recent Updates
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FB2013_03	Stocks FBst0043658
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Geyer and Corces, 1987, Benedyk et al., 1994, Morcillo et al., 1996)
Uses	CV term Qualifiers & info Reference characterization y (Geyer and Corces, 1987, Benedyk et al., 1994) rescue construct y (Benedyk et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	31.313
Left end	P5\' (Geyer and Corces, 1987, Huet et al., 1999.1.7)
Right end	P3\' (Geyer and Corces, 1987, Huet et al., 1999.1.7)

Segments	Number Orientation Symbol 1 + Car1_P5'(P5',R1) 2 + 001014 2 + 001305 3 - y⁺7.6(S,Bg2) 3 - y⁺3'(0.15) 4 + 001015 4 + BglII 5 + Car20_P3'(H3,P3') 5 - y⁺7.6(S,Bg2) 6 + 001015 7 + Car20_P3'(H3,P3')
Reference	(Geyer and Corces, 1987, Huet et al., 1999.1.7)
Features
	CV term Qualifiers & info Reference complete rescue (Spradling and Rubin, 1983) complete rescue (Basler and Struhl, 1994) complete rescue (Benedyk et al., 1994) selectable marker (Rubin and Spradling, 1983, Geyer and Corces, 1987, Xu and Rubin, 1993, Brower et al., 1995, Morcillo et al., 1996) selectable marker (Morcillo et al., 1996) selectable marker (Geyer and Corces, 1987, Xu and Rubin, 1993, Morcillo et al., 1996, Harrison et al., 1989, Xu et al., 1995, Keeler and Gloor, 1997, Huet et al., 1999.1.7) visible marker (Geyer and Corces, 1987)
Component Alleles
Allele	ry^+t7.2
Reference(s)	(Rubin and Spradling, 1983, Xu and Rubin, 1993, Brower et al., 1995, Geyer and Corces, 1987, Harrison et al., 1989, Morcillo et al., 1996)
Molecular data	Construct: 7.2kb HindIII fragment that contains ry⁺ function. (Rubin and Spradling, 1983)
Phenotypic class	wild-type (Rubin and Spradling, 1983)
Phenotype manifest in
Other information

Allele	y^+t7.7
Reference(s)	(Basler and Struhl, 1994, Geyer and Corces, 1987, Xu and Rubin, 1993, Xu et al., 1995, Newsome et al., 2000, Harrison et al., 1989, Huet et al., 1999.1.7, Morcillo et al., 1996, Keeler and Gloor, 1997, Benedyk et al., 1994)
Molecular data	Construct: 8kb SalI fragment carrying y⁺. (Merriam, 1997.5.9) Contained within 7.7kb SalI-BglII fragment that includes the entire y⁺ gene. (Struhl and Basler, 1993) Fragment containing the y gene, including 2873bp 5' and 130bp 3' flanking sequences. (Harrison et al., 1989) 5' regulatory region deletion derivative of y, 2873 nucleotides remain upstream of the transcription start site. (Geyer and Corces, 1987)
Phenotypic class	wild-type (Benedyk et al., 1994, Geyer and Corces, 1987, Harrison et al., 1989)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{Car20y} (Geyer and Corces, 1987)
descendant(s)	P{CaryP} (Fish and Groth, 2005.2.4)
Comments
	sequence comment: Included as non-attributed correction. (Geyer, 1999.1.9, Nassif et al., 1994) construct_comment: he y⁺ fragment in this construct is frequently isolated using the 5\' SalI site and the polylinker SalI site 3\'. This SalI fragment is comprised of 7.7kb of contiguous y⁺ genomic sequences plus 150bp of sequence derived from a region 1.7kb further downstream. Included as non-attributed correction. (Geyer, 1999.1.9) report comment: (Geyer, 1999.1.9, Nassif et al., 1994) mapping_comment: Included as non-attributed correction. (Geyer, 1999.1.9, Nassif et al., 1994) construct_comment: Largest of a series of constructs used for deletion analysis of the 5\' regulatory region of the yellow gene. (Geyer and Corces, 1987)
Stocks Listed in FlyBase ( 1385 )
Bloomington	1816 y¹ w¹¹¹⁸; P{Car20y}25F P{neoFRT}40A 2118 y¹ w¹¹¹⁸; P{neoFRT}42D P{Car20y}44B 1295 y¹ w^*; sna^Sco/CyO, P{Car20y}EW1 1942 y¹ w¹¹¹⁸; P{Car20y}66E P{neoFRT}73D P{neoFRT}80B 10470 y¹ w^67c23; P{lacW}AGO1^k00208/CyO, P{Car20y}EW1
Kyoto	106519 y¹ w¹¹¹⁸; P{Car20y}25F P{neoFRT}40A 106668 y¹ w¹¹¹⁸; P{neoFRT}42D P{Car20y}44B 101879 y^* w¹¹¹⁸; P{Car20y}25F P{neoFRT}40A 101878 y¹ w¹¹¹⁸ ; P{neoFRT}42D P{Car20y}44B 108945 y¹ w^; CyO, P{Car20y}EW1/sna^Sco 106591 y¹ w¹¹¹⁸; P{Car20y}66E P{neoFRT}73D P{neoFRT}80B 101928 y^ w¹¹¹⁸ ; P{Car20y}66E P{neoFRT}73D P{neoFRT}80B
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External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	D-2873 (Nassif et al., 1994, Geyer and Corces, 1987) P[y⁺] (Newsome et al., 2000) P{Car20y} (FlyBase, 1996-, Geyer, 1999.1.9, FlyBase, 1992-) P{y⁺} (FlyBase, 1996-) P{y^+t2873 ry^+t7.2=Car20y} (FlyBase, 1996-, Geyer and Corces, 1987)
Secondary FlyBase IDs
	FBtp0000148 FBtp0003590
References ( 15 )
Research paper	Monge et al., 2001, Development 128(8): 1239--1252 Drosophila transcription factor AP-2 in proboscis, leg and brain central complex development. [FBrf0134567] Newsome et al., 2000, Development 127(4): 851--860 Analysis of Drosophila photoreceptor axon guidance in eye-specific mosaics. [FBrf0125463] Keeler and Gloor, 1997, Mol. Cell. Biol. 17(2): 627--634 Efficient gap repair in Drosophila melanogaster requires a maximum of 31 nucleotides of homologous sequence at the searching ends. [FBrf0091098] Morcillo et al., 1996, J. Hered. 87(5): 399--403 The use of transposable P-elements of Drosophila melanogaster for introductory genetics laboratory courses. [FBrf0090703] Brower et al., 1995, Development 121(5): 1311--1320 Nonequivalent requirements for PS1 and PS2 integrin at cell attachments in Drosophila: genetic analysis of the a_PS1 integrin subunit. [FBrf0078125] Xu et al., 1995, Development 121(4): 1053--1063 Identifying tumor suppressors in genetic mosaics: the Drosophila lats gene encodes a putative protein kinase. [FBrf0079656] Benedyk et al., 1994, Genes Dev. 8(1): 105--117 odd-paired: a zinc finger pair-rule protein required for the timely activation of engrailed and wingless in Drosophila embryos. [FBrf0068410] Nassif et al., 1994, Mol. Cell. Biol. 14(3): 1613--1625 Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair. [FBrf0068604] Xu and Rubin, 1993, Development 117(4): 1223--1237 Analysis of genetic mosaics in developing and adult Drosophila tissues. [FBrf0058070] Harrison et al., 1989, Dev. Genet. 10: 239--248 The gypsy retrotransposon of Drosophila melanogaster: mechanisms of mutagenesis and interaction with the suppressor of Hairy-wing locus. [FBrf0049522] Geyer and Corces, 1987, Genes Dev. 1: 996--1004 Separate regulatory elements are responsible for the complex pattern of tissue-specific and developmental transcription of the yellow locus in Drosophila melanogaster. [FBrf0046293]
Personal communication to FlyBase	Fish and Groth, 2005.2.4, Progenitor of P{CARY.attP}. Progenitor of P{CARY.attP}. [FBrf0182785] Geyer, 1999.1.9, yellow constructs -- extra fragment. yellow constructs -- extra fragment. [FBrf0105494] Huet et al., 1999.1.7, pc regarding yellow constructs. pc regarding yellow constructs. [FBrf0105493]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]