Recombinant construct M{nos-int.NLS}

General Information
Symbol	M{nos-int.NLS}	FlyBase ID	FBtp0022218
Feature type	transgenic_transposon

Size		Expression data	GFP reporter
Associated insertions	2 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	Drosophila melanogaster
Location-dependent role
Description	CV term Qualifiers & info Reference transposon Dmau\mariner (Bischof et al., 2007) transposon_modified_in_vivo
Uses	CV term Qualifiers & info Reference characterization nos (Bischof et al., 2007)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Bischof et al., 2007) selectable marker (Bischof et al., 2007) selectable marker (Bischof et al., 2007)
Component Alleles
Allele	Avic\GFP^E.3xP3
Reference(s)	(Bischof et al., 2007)
Molecular data	Three tandem repeats of an artificially derived Mmus\Pax6 homodimer binding site are fused upstream of a TATA box driving expression of "EGFP". (Horn et al., 2000) Construct: Artificial promoter containing three binding sites for Mmus\Pax6 homodimers in front of a TATA box drives the expression of the enhanced variant of Avic\GFP (EGFP). (Berghammer et al., 1999)
Phenotypic class
Phenotype manifest in
Other information

Allele	Disc\RFP^3xP3.PB
Reference(s)	(Bischof et al., 2007)
Molecular data	An artificial "3xP3" promoter drives expression of Disc\RFP. The construct has a αTub84B 3' UTR. (Bischof et al., 2007)
Phenotypic class
Phenotype manifest in
Other information

Allele	phiC31\int^{nos.T:SV40\nls2}
Reference(s)	(Bischof et al., 2007)
Molecular data	A nos promoter drives expression of phiC31\int, which is tagged with T:SV40\nls2. The phiC31\int construct also contains nos 3'UTR sequences. (Bischof et al., 2007)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 16 -- 17 Bolwig organ \| presumptive (Horn et al., 2000) larval stage embryonic/larval sense organ (Horn et al., 2000) anal pad (Horn et al., 2000) embryonic/larval central nervous system (Horn et al., 2000) Bolwig organ (Horn et al., 2000) embryonic/larval hindgut (Horn et al., 2000) pupal stage \| late eye (Horn et al., 2000) adult stage eye (Horn et al., 2000) ocellus (Horn et al., 2000)
Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	M{3xP3-RFP.attP} (Bischof et al., 2007) p3xP3-EGFP.nos-int.NLS.attB (Bischof et al., 2007)
descendant(s)
Comments
	Generated by phiC31\int-mediated integration of p3xP3-EGFP.nos-int.NLS.attB into a target "landing site" insertion of the M{3xP3-RFP.attP} construct. (Bischof et al., 2007) The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP^3xP3.PB marker, followed by another P1\loxP site, followed by a Avic\GFP^E.3xP3 marker, followed by another P1\loxP site, followed by phiC31\int^{nos.T:SV40\nls2}, which provides the integrase source. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP^3xP3.PB and Avic\GFP^E.3xP3 markers by cre-mediated recombination. (Bischof et al., 2007)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	M{nos-int.NLS} (FlyBase, 1996-) nos-phi^NLS (Bischof et al., 2007)
Secondary FlyBase IDs

References ( 2 )
Research paper	Bischof et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(9): 3312--3317 An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases. [FBrf0192974]
Supplementary material	Bischof et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(9): SI Figure 4. [FBrf0198669]