Recombinant construct M{vas-int.B}

General Information
Symbol	M{vas-int.B}	FlyBase ID	FBtp0022219
Feature type	transgenic_transposon	Created / Updated	2007-06-14/2007-06-14

Size		Expression data	GFP reporter
Location-dependent role		Associated insertions	2 available
Molecular map
Description & Uses
Species	Drosophila melanogaster
Description	CV term Qualifiers & info Reference transposon_modified_in_vivo
Uses	CV term Qualifiers & info Reference integrase source recombination-mediated genomic insertion \| phiC31\int (Bischof et al., 2007)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Bischof et al., 2007) selectable marker (Bischof et al., 2007)
Component Alleles
Disc\RFP^3xP3.PB
Reference(s)	(Bischof et al., 2007)
Molecular data	An artificial "3xP3" promoter drives expression of Disc\RFP. The construct has a αTub84B 3' UTR. (Bischof et al., 2006)
Phenotypic class
Phenotype manifest in
Other information
Avic\GFP^E.3xP3
Reference(s)	(Bischof et al., 2007)
Molecular data	Construct: Artificial promoter containing three binding sites for Mmus\Pax6 homodimers in front of a TATA box drives the expression of the enhanced variant of Avic\GFP (EGFP). (Berghammer et al., 1999) Three tandem repeats of an artificially derived Mmus\Pax6 homodimer binding site are fused upstream of a TATA box driving expression of "EGFP". (Horn et al., 2000)
Phenotypic class
Phenotype manifest in
Other information
phiC31\int^vas.PB
Reference(s)	(Bischof et al., 2007)
Molecular data	A vas promoter drives expression of phiC31\int. The construct also has vas 3'UTR sequences. (Bischof et al., 2006)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference adult stage ocellus (Horn et al., 2000) larval stage anal pad (Horn et al., 2000) larval stage larval central nervous system (Horn et al., 2000) larval stage larval sense organ (Horn et al., 2000) pupal stage \| late eye (Horn et al., 2000) larval stage Bolwig\\\'s organ (Horn et al., 2000) larval stage larval hindgut (Horn et al., 2000) adult stage eye (Horn et al., 2000) embryonic stage \| stage >=16 Bolwig\\\'s organ \| presumptive (Horn et al., 2000)
Additional Information
Statement Reference

Assay mode	distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000) distribution deduced from reporter (Horn et al., 2000)
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	M{3xP3-RFP.attP} p3xP3-EGFP.vas-int.attB (Bischof et al., 2007, Bischof et al., 2007)
descendant(s)
Comments
	Generated by phiC31\int-mediated integration of p3xP3-EGFP.vas-int.attB into a target "landing site" insertion of the M{3xP3-RFP.attP} construct. (Bischof et al., 2007) The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP[3xP3.PB] marker, followed by another P1\loxP site, followed by a Avic\GFP[E.3xP3] marker, followed by another P1\loxP site, followed by phiC31\int[vas.PB], which provides the integrase source. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP[3xP3.PB] and Avic\GFP[E.3xP3] markers by cre-mediated recombination. (Bischof et al., 2007)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	M{vas-int.B} (FlyBase, 1996-) vas-phi (Bischof et al., 2007)
Secondary FlyBase IDs

References ( 6 )
Research paper	Bischof et al., 2007, Proc. Natl. Acad. Sci. USA 104(9): 3312--3317 An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases. [FBrf0192974] Horn et al., 2000, Dev. Genes Evol. 210(12): 623--629 Highly sensitive, fluorescent transformation marker for Drosophila transgenesis. [FBrf0132292] Berghammer et al., 1999, Nature 402(6760): 370--371 A universal marker for transgenic insects. [FBrf0122963]
Supplementary material	Bischof et al., 2006, Proc. Natl. Acad. Sci. USA 104(9): SI Figure 4. [FBrf0198669] Bischof et al., 2006, Proc. Natl. Acad. Sci. USA 104(9): SI Text. [FBrf0198668]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]