Recombinant construct P{VGlut-GAL4.D}

General Information
Symbol	P{VGlut-GAL4.D}	FlyBase ID	FBtp0022557
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	4 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Collins et al., 2006)
Uses	CV term Qualifiers & info Reference binary system (regulatory) VGlut (Collins et al., 2006)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Collins et al., 2006)
Component Alleles
Allele	Scer\GAL4^VGlut.PD
Reference(s)	(Collins et al., 2006, Daniels et al., 2008, Graf et al., 2009, Grygoruk et al., 2010, Daniels et al., 2011, Chiang et al., 2011, Raghu and Borst, 2011, Kahsai and Winther, 2011, Carlsson et al., 2010, Graf et al., 2012, Kahsai et al., 2012)
Molecular data	5.5kb fragment from immediately upstream of the VGlut translation start site is fused upstream of the Scer\GAL4 gene. (Daniels et al., 2008)
Phenotypic class	flightless, with VGlut^Scer\UAS.cDa (Daniels et al., 2011) gravitaxis defective, with VGlut^Scer\UAS.cDa (Daniels et al., 2011) lethal, with VGlut^Scer\UAS.cDa (Daniels et al., 2011) lethal, with VGlut^{Scer\UAS.T:Avic\GFP-EGFP} (Daniels et al., 2011) lethal \| pupal stage, with VGlut^{Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) neuroanatomy defective, with VGlut^{Scer\UAS.T:Avic\GFP-EGFP} (Daniels et al., 2011) neurophysiology defective, with VGlut^Scer\UAS.cDa (Daniels et al., 2011) uncoordinated, with VGlut^Scer\UAS.cDa (Daniels et al., 2011) viable, with VGlut^{B1.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B2.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B3.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B4.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B5.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B7.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B9.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^B10.Scer\UAS (Grygoruk et al., 2010) viable, with VGlut^{B11.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B13.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B15.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B16.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B18.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B19.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B20.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B21.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B22.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B23.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B24.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B25.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B26.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B27.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B28.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B29.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B30.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^B31.Scer\UAS (Grygoruk et al., 2010) viable, with VGlut^{B32.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B33.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B34.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^B35.Scer\UAS (Grygoruk et al., 2010) viable, with VGlut^{B38.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B39.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B40.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B41.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B42.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B43.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B44.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B45.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B46.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B47.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B48.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B49.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B50.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{B52.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{RD7.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010) viable, with VGlut^{RD16.Scer\UAS.T:Avic\GFP-EGFP} (Grygoruk et al., 2010)
Phenotype manifest in	antennal lobe, with VGlut^{Scer\UAS.T:Avic\GFP-EGFP} (Daniels et al., 2011) neuropil, with VGlut^{Scer\UAS.T:Avic\GFP-EGFP} (Daniels et al., 2011)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference third instar larval stage embryonic/larval neuromuscular junction of glutamatergic neuron (Daniels et al., 2008) embryonic/larval neuromuscular junction \| restricted (Graf et al., 2009) Comment:Presynaptic wandering third instar larval stage glutamatergic neuron of ventral nerve cord (Daniels et al., 2008) embryonic/larval optic lobe (Daniels et al., 2008) adult stage mushroom body spur \| faint (Daniels et al., 2008) mushroom body alpha-lobe \| faint (Daniels et al., 2008) mushroom body calyx \| faint (Daniels et al., 2008) adult antennal lobe (Daniels et al., 2008) local interneuron of adult antennal lobe \| subset (Daniels et al., 2008) adult antennal lobe projection neuron \| subset (Daniels et al., 2008) motor neuron \| subset (Daniels et al., 2008) ellipsoid body (Daniels et al., 2008, Chen et al., 2012) ring neuron (Daniels et al., 2008) fan-shaped body (Daniels et al., 2008, Kahsai et al., 2012) nodulus (Daniels et al., 2008) protocerebral bridge (Daniels et al., 2008) protocerebral bridge glomerulus 8 \| faint (Daniels et al., 2008) optic lobe (Daniels et al., 2008) medulla (Daniels et al., 2008) lobula plate (Daniels et al., 2008) superior medial protocerebrum (Daniels et al., 2008) ventrolateral protocerebrum (Daniels et al., 2008) adult subesophageal ganglion (Daniels et al., 2008) lateral horn (Daniels et al., 2008) glutamatergic neuron (Wu et al., 2010) neuron \| subset of adult brain (Chen et al., 2012) adult mushroom body (Chen et al., 2012) Comment:faint expression eye (Chen et al., 2012) Comment:medium expression antennal lobe projection neuron (Chen et al., 2012) Comment:faint expression lamina \| restricted (Raghu and Borst, 2011) medulla \| restricted (Raghu and Borst, 2011) lobula \| restricted (Raghu and Borst, 2011) lobula plate \| restricted (Raghu and Borst, 2011) lamina monopolar neuron L2 (Raghu and Borst, 2011) transmedullary neuron Tm9 (Raghu and Borst, 2011) transmedullary neuron Tm20 (Raghu and Borst, 2011) transmedullary neuron vGlutTmnew1 (Raghu and Borst, 2011) transmedullary Y neuron vGlutTmYnew1 (Raghu and Borst, 2011) Y neuron Y1 (Raghu and Borst, 2011) Y neuron vGlutYnew1 (Raghu and Borst, 2011) medullary intrinsic neuron Mi4 (Raghu and Borst, 2011) medullary intrinsic neuron vGlutMinew1 (Raghu and Borst, 2011) medullary intrinsic neuron vGlutMinew2 (Raghu and Borst, 2011) distal medullary amacrine neuron Dm3 (Raghu and Borst, 2011) distal medullary amacrine neuron Dm5 (Raghu and Borst, 2011) distal medullary amacrine neuron vGlutDmnew1 (Raghu and Borst, 2011) proximal medullary amacrine neuron Pm2 (Raghu and Borst, 2011) medullary intrinsic amacrine neuron vGlutMi Amnew1 (Raghu and Borst, 2011) medullary tangential neuron Mt11 (Raghu and Borst, 2011) T neuron T3 (Raghu and Borst, 2011) T neuron T5 (Raghu and Borst, 2011) translobula plate neuron Tlp1 (Raghu and Borst, 2011) translobula plate neuron Tlp3 (Raghu and Borst, 2011) translobula plate neuron vGlutTlpnew1 (Raghu and Borst, 2011) lobula columnar neuron Lcn4 (Raghu and Borst, 2011) lobula columnar neuron Lcn5 (Raghu and Borst, 2011) lobula columnar neuron Lcn8 (Raghu and Borst, 2011) lobula tangential neuron Lt4 (Raghu and Borst, 2011) lobula tangential neuron Lt6 (Raghu and Borst, 2011) lobula intrinsic neuron Li1 (Raghu and Borst, 2011) ventral giant horizontal cell HSS (Raghu and Borst, 2011) lobula plate intrinsic neuron LPi1 (Raghu and Borst, 2011) ellipsoid body outer ring \| restricted (Kahsai et al., 2012) ellipsoid body posterior shell \| restricted (Kahsai et al., 2012) protocerebral bridge \| restricted (Kahsai et al., 2012) bulb \| restricted (Kahsai et al., 2012) nodulus \| restricted (Kahsai et al., 2012) adult mushroom body gamma-lobe \| faint (Daniels et al., 2008)
Additional Information
Statement Reference Scer\GAL4[VGlut.PD] is labels a subset of glutamatergic neurons in the medulla, lamina, lobula and lobula plate. (Raghu and Borst, 2011) Scer\GAL4[VGlut.PD] drives expression in the ellipsoid body rings in the innervation regions of R3 and R4d ring neurons and in a ring proximal to the R3 ring. In the fan-shaped body strong expression is detected in the central layers with weaker signal in the dorsal and ventral layers. Expression is also observed in the nodulus and in the protocerebral bridge (except for two segments on each lateral side). Co-localisation with mGluRA protein is observed in the ellipsoid body rings R3 and R4d, in the fan-shaped body, nodulus and protocerebral bridge. (Kahsai et al., 2012) Expression of Scer\GAL4[VGlut.PD] is seen in many, but not all, glutamatergic neurons of the larva, and a few apparently non-glutamatergic neurons. In the adult brain, expression of VGlut shows extensive expression in central neuropil compartments, but is predominantly absent from the mushroom body only being detectable as punctae in the base of the gamma-lobes, the tip but not the base of the alpha-lobe (in contrast to VGlut which is seen in the base), the spur, and faintly in the calyx. Expression of Scer\GAL4[VGlut.PD] does not perfectly reflect expression of VGlut. Expression in the adult brain is seen in a few motor neurons that innervate head muscles. Expression within the ellipsoid body is observed within its inner and outer layers, a pattern suggestive of R-cell innervation. The expression seen in the protocerebral bridge is extensive across all glomeruli, but is weaker in the most distal glomerulus - glomerulus number 8. Expression in the adult optic lobe is strongest in an outer medulla layer and the inner medulla. (Daniels et al., 2008)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	24635 P{VGlut-GAL4.D}1, w^*
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	dvglut^CNSIII-Gal4 (Daniels et al., 2011) dvGlut^CNSIII-Gal4 (Raghu and Borst, 2011) dvglut^CNSIII GAL4 (Kahsai and Winther, 2011) dvglut^NMJX-Gal4 (Graf et al., 2009, Graf et al., 2012) DVGlut-GAL4 (Collins et al., 2006) dvglut-Gal4 (Daniels et al., 2008, Carlsson et al., 2010) dVGLUT-GAL4 (Grygoruk et al., 2010) dvGluT GAL4 (Kahsai and Winther, 2011) dvGLuT-GAL4 (Kahsai and Winther, 2011) dvglut-GAL4 (Carlsson et al., 2010) P{VGlut-GAL4.D} (FlyBase, 1996-) P{VGlut-GAL4} (FlyBase, 1992-) vesicular glutamate transporter GAL4 (Kahsai and Winther, 2011) VGlut-Gal4 (Chiang et al., 2011) VGlut-GAL4 (Chiang et al., 2011) vGluT-GAL4 (Kahsai et al., 2012) vglut-GAL4 (Kahsai and Winther, 2011)
Secondary FlyBase IDs

References ( 13 )
Research paper	Graf et al., 2012, J. Neurosci. 32(47): 16586--16596 RIM Promotes Calcium Channel Accumulation at Active Zones of the Drosophila Neuromuscular Junction. [FBrf0220028] Kahsai et al., 2012, Neuroscience 208: 11--26 Distribution of metabotropic receptors of serotonin, dopamine, GABA, glutamate, and short neuropeptide F in the central complex of Drosophila. [FBrf0217851] Chiang et al., 2011, Curr. Biol. 21(1): 1--11 Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution. [FBrf0212704] Daniels et al., 2011, Neurobiol. Disease 41(2): 415--420 Increased vesicular glutamate transporter expression causes excitotoxic neurodegeneration. [FBrf0212635] Kahsai and Winther, 2011, J. Comp. Neurol. 519(2): 290--315 Chemical neuroanatomy of the Drosophila central complex: distribution of multiple neuropeptides in relation to neurotransmitters. [FBrf0212649] Raghu and Borst, 2011, PLoS ONE 6(5): e19472 Candidate glutamatergic neurons in the visual system of Drosophila. [FBrf0213690] Carlsson et al., 2010, J. Comp. Neurol. 518(16): 3359--3380 Multiple neuropeptides in the Drosophila antennal lobe suggest complex modulatory circuits. [FBrf0211156] Grygoruk et al., 2010, J. Biol. Chem. 285(10): 6867--6878 A Tyrosine-based Motif Localizes a Drosophila Vesicular Transporter to Synaptic Vesicles in Vivo. [FBrf0210098] Graf et al., 2009, Neuron 64(5): 663--677 Rab3 Dynamically Controls Protein Composition at Active Zones. [FBrf0209504] Daniels et al., 2008, J. Comp. Neurol. 508(1): 131--152 Visualizing glutamatergic cell bodies and synapses in Drosophila larval and adult CNS. [FBrf0204872] Collins et al., 2006, Neuron 51(1): 57--69 Highwire restrains synaptic growth by attenuating a MAP kinase signal. [FBrf0194919]
Personal communication to FlyBase	Saxton, 2007.12.13, P{VGlut-GAL4}1. P{VGlut-GAL4}1. [FBrf0200383]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]