Recombinant construct P{UAS-brp.GFP}

General Information
Symbol	P{UAS-brp.GFP}	FlyBase ID	FBtp0022731
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	3 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Wagh et al., 2006)
Uses	CV term Qualifiers & info Reference binary system (targeted) brp (Wagh et al., 2006) characterization brp (Wagh et al., 2006) reporter construct vital \| brp (Wagh et al., 2006)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Wagh et al., 2006) molecular tag (Wagh et al., 2006) selectable marker (Wagh et al., 2006)
Component Alleles
Allele	brp^{Scer\UAS.T:Avic\GFP}
Reference(s)	(Wagh et al., 2006, Pitman et al., 2011, Johnson et al., 2009, Ataman et al., 2008, Boerner and Duch, 2010, Tsurudome et al., 2010, Han et al., 2011)
Molecular data	Scer\UAS regulatory sequences drive expression of the 5'UTR and coding regions of brp, which is tagged with T:Avic\GFP at the N-terminus. The brp coding sequences correspond to cDNA AT09405. (Wagh et al., 2006)
Phenotypic class	neurophysiology defective, with Scer\GAL4^BG380 (Tsurudome et al., 2010) neurophysiology defective, with Scer\GAL4^{elav.Switch.PO} (Tsurudome et al., 2010)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Wagh et al., 2006)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 3 )
Bloomington	35848 P{UAS-brp.GFP}1, w^* 36291 w^; P{UAS-brp.GFP}TR722 36292 w^; P{UAS-brp.GFP}TR725
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	GFP-tagged bruchpilot (Boerner and Duch, 2010) P{UAS-brp.GFP} (FlyBase, 1992-) UAS-Brp (Tsurudome et al., 2010) uas-Brp::GFP (Pitman et al., 2011) UAS-Brp-GFP (Ataman et al., 2008, Han et al., 2011) UAS-g-brp (Johnson et al., 2009) UAS-gfp-brp (Johnson et al., 2009)
Secondary FlyBase IDs

References ( 9 )
Research paper	Han et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(23): 9673--9678 Enhancer-driven membrane markers for analysis of nonautonomous mechanisms reveal neuron-glia interactions in Drosophila. [FBrf0213916] Pitman et al., 2011, Curr. Biol. 21(10): 855--861 A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body. [FBrf0213725] Boerner and Duch, 2010, J. Comp. Neurol. 518(13): 2437--2455 Average shape standard atlas for the adult Drosophila ventral nerve cord. [FBrf0210897] Tsurudome et al., 2010, Neuron 68(5): 879--893 The Drosophila miR-310 Cluster Negatively Regulates Synaptic Strength at the Neuromuscular Junction. [FBrf0212495] Johnson et al., 2009, PLoS Biol. 7(9): e1000193 Negative regulation of active zone assembly by a newly identified SR protein kinase. [FBrf0208771] Ataman et al., 2008, Neuron 57(5): 705--718 Rapid activity-dependent modifications in synaptic structure and function require bidirectional Wnt signaling. [FBrf0204152] Wagh et al., 2006, Neuron 49(6): 833--844 Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila. [FBrf0191329]
Personal communication to FlyBase	Han and Jan, 2011.6.28, P{UAS-brp.GFP}1. P{UAS-brp.GFP}1. [FBrf0214033] Sigrist, 2011.7.29, P{UAS-brp.GFP} insertions. P{UAS-brp.GFP} insertions. [FBrf0214582]