Recombinant construct M{nos-int.B}

General Information
Symbol	M{nos-int.B}	FlyBase ID	FBtp0023081
Feature type	transgenic_transposon

Size		Expression data	GFP reporter
Associated insertions	3 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon Dmau\mariner (Bischof et al., 2007) transposon_modified_in_vivo
Uses	CV term Qualifiers & info Reference characterization nos (Bischof et al., 2007) integrase source recombination-mediated genomic insertion \| phiC31\int (Bischof et al., 2007)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Bischof et al., 2007) selectable marker (Bischof et al., 2007)
Component Alleles
Allele	Avic\GFP^E.3xP3
Reference(s)	(Bischof et al., 2007)
Molecular data	Three tandem repeats of an artificially derived Mmus\Pax6 homodimer binding site are fused upstream of a TATA box driving expression of "EGFP". (Horn et al., 2000) Construct: Artificial promoter containing three binding sites for Mmus\Pax6 homodimers in front of a TATA box drives the expression of the enhanced variant of Avic\GFP (EGFP). (Berghammer et al., 1999)
Phenotypic class
Phenotype manifest in
Other information

Allele	Disc\RFP^3xP3.PB
Reference(s)	(Bischof et al., 2007)
Molecular data	An artificial "3xP3" promoter drives expression of Disc\RFP. The construct has a αTub84B 3' UTR. (Bischof et al., 2007)
Phenotypic class
Phenotype manifest in
Other information

Allele	phiC31\int^nos.PB
Reference(s)	(Bischof et al., 2007)
Molecular data	A nos promoter drives expression of phiC31\int. The construct also has nos 3'UTR sequences. (Bischof et al., 2007)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 16 -- 17 Bolwig organ \| presumptive (Horn et al., 2000) larval stage embryonic/larval sense organ (Horn et al., 2000) anal pad (Horn et al., 2000) embryonic/larval central nervous system (Horn et al., 2000) Bolwig organ (Horn et al., 2000) embryonic/larval hindgut (Horn et al., 2000) pupal stage \| late eye (Horn et al., 2000) adult stage eye (Horn et al., 2000) ocellus (Horn et al., 2000)
Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	M{3xP3-RFP.attP} (Bischof et al., 2007) p3xP3-EGFP.nos-int.attB (Bischof et al., 2007)
descendant(s)
Comments
	Generated by phiC31\int-mediated integration of p3xP3-EGFP.nos-int.attB into a target "landing site" insertion of the M{3xP3-RFP.attP} construct. (Bischof et al., 2007) The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP^3xP3.PB marker, followed by another P1\loxP site, followed by a Avic\GFP^E.3xP3 marker, followed by another P1\loxP site, followed by phiC31\int^nos.PB, which provides the integrase source. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP^3xP3.PB and Avic\GFP^E.3xP3 markers by cre-mediated recombination. (Bischof et al., 2007)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	M{nos-int.B} (FlyBase, 1996-) nos-phi (Bischof et al., 2007)
Secondary FlyBase IDs

References ( 2 )
Research paper	Leonardi et al., 2011, Development 138(16): 3569--3578 Multiple O-glucosylation sites on Notch function as a buffer against temperature-dependent loss of signaling. [FBrf0214548] Bischof et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(9): 3312--3317 An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases. [FBrf0192974]