Recombinant construct M{w^Ex3-6.w^P-Ex2}

General Information
Symbol	M{w^Ex3-6.w^P-Ex2}	FlyBase ID	FBtp0023084
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon transposon_modified_in_vivo
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Bischof et al., 2007) selectable marker (Bischof et al., 2007)
Component Alleles
Allele	Disc\RFP^3xP3.PB
Reference(s)	(Bischof et al., 2007)
Molecular data	An artificial "3xP3" promoter drives expression of Disc\RFP. The construct has a αTub84B 3' UTR. (Bischof et al., 2007)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^Ex3-6
Reference(s)	(Bischof et al., 2007)
Molecular data	Contains exon 3-6, introns 3-5, and the 57 bp of intron 2 next to exon 3 from w. (Bischof et al., 2007)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^P-Ex2
Reference(s)	(Bischof et al., 2007)
Molecular data	Contains the promoter, exons 1 and 2, intron 1 and the first 17 nucleotides of intron 2 from the w gene. (Bischof et al., 2007)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	M{w^Ex3-6.attP} (Bischof et al., 2007) pw^P-Ex2UASTattB (Bischof et al., 2007)
descendant(s)
Comments
	This construct is expected to allow expression of a functional w gene. (Bischof et al., 2007) Generated by phiC31\int-mediated integration of pw^P-Ex2UASTattB into a target "landing site" insertion of the M{w^Ex3-6.attP} construct. (Bischof et al., 2007) The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP^3xP3.PB marker, followed by another P1\loxP site, followed by w^Ex3-6, followed by w^P-Ex2, followed by another P1\loxP site, followed by a Scer\UAS multiple cloning site. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP^3xP3.PB, w^Ex3-6 and w^Ex3-6 by cre-mediated recombination. (Bischof et al., 2007)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	M{w^Ex3-6.w^P-Ex2} (FlyBase, 1996-) unnamed (Bischof et al., 2007)
Secondary FlyBase IDs

References ( 1 )
Research paper	Bischof et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(9): 3312--3317 An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases. [FBrf0192974]

Recombinant construct M{wEx3-6.wP-Ex2}

Recombinant construct M{w^Ex3-6.w^P-Ex2}