Recombinant construct P{GD13500}

General Information
Symbol	P{GD13500}	FlyBase ID	FBtp0026452
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms large body
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Associated Sequence Features	dsRNA-GD13500
Description	CV term Qualifiers & info Reference transposon P-element (Dickson et al., 2007.7.18)
Uses	CV term Qualifiers & info Reference binary system (targeted) Pten (Dickson et al., 2007.7.18) characterization Pten (Dickson et al., 2007.7.18) RNAi (dsRNA) construct Pten (Dickson et al., 2007.7.18)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Dickson et al., 2007.7.18)
Component Alleles
Allele	Pten^GD13500
Reference(s)	(Dickson et al., 2007.7.18, Resnik-Docampo and de Celis, 2011, Mummery-Widmer et al., 2009, Gault et al., 2012, Okamoto et al., 2013)
Molecular data	Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Phenotypic class	immune response defective \| larval stage, with Scer\GAL4^Hml.PG (Howell et al., 2012) increased cell number, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) large body \| pupal stage, with Scer\GAL4^tub.PU (Okamoto et al., 2013) partially lethal - majority die \| pupal stage, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) size defective, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype manifest in	chaeta \| supernumerary, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) wing, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
VDRC	v35731 w¹¹¹⁸; P{GD13500}v35731
External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 13500
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{GD13500} (FlyBase, 1996-, Dickson et al., 2007.7.18) UAS-iPTEN (Resnik-Docampo and de Celis, 2011) UAS-PTEN^IR (Gault et al., 2012) UAS-PTEN-RNAi (Okamoto et al., 2013)
Secondary FlyBase IDs

References ( 6 )
Research paper	Okamoto et al., 2013, Genes Dev. 27(1): 87--97 A secreted decoy of InR antagonizes insulin/IGF signaling to restrict body growth in Drosophila. [FBrf0220509] Gault et al., 2012, J. Cell Biol. 196(5): 605--621 Drosophila CK1-γ, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. [FBrf0217630] Resnik-Docampo and de Celis, 2011, PLoS ONE 6(1): e14528 MAP4K3 Is a Component of the TORC1 Signalling Complex that Modulates Cell Growth and Viability in Drosophila melanogaster. [FBrf0212911]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Dietzl et al., 2007, Nature 448(7150): Supplementary Table 1. [FBrf0202579]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]