Recombinant construct M{vas-int.Dm}

General Information
Symbol	M{vas-int.Dm}	FlyBase ID	FBtp0036669
Feature type	transgenic_transposon

Size		Expression data	GFP reporter
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon Dmau\mariner (Bischof and Basler, 2007.11.7, Mishra et al., 2010) transposon_modified_in_vivo
Uses	CV term Qualifiers & info Reference integrase source recombination-mediated genomic insertion \| phiC31\int (Bischof and Basler, 2007.11.7)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Bischof and Basler, 2007.11.7) selectable marker (Mishra et al., 2010) selectable marker (Bischof and Basler, 2007.11.7) selectable marker (Mishra et al., 2010)
Component Alleles
Allele	Avic\GFP^E.3xP3
Reference(s)	(Bischof and Basler, 2007.11.7, Mishra et al., 2010)
Molecular data	Three tandem repeats of an artificially derived Mmus\Pax6 homodimer binding site are fused upstream of a TATA box driving expression of "EGFP". (Horn et al., 2000) Construct: Artificial promoter containing three binding sites for Mmus\Pax6 homodimers in front of a TATA box drives the expression of the enhanced variant of Avic\GFP (EGFP). (Berghammer et al., 1999)
Phenotypic class
Phenotype manifest in
Other information

Allele	Disc\RFP^3xP3.PB
Reference(s)	(Bischof and Basler, 2007.11.7)
Molecular data	An artificial "3xP3" promoter drives expression of Disc\RFP. The construct has a αTub84B 3' UTR. (Bischof et al., 2007)
Phenotypic class
Phenotype manifest in
Other information

Allele	phiC31\int^Dm.vas
Reference(s)	(Bischof and Basler, 2007.11.7)
Molecular data	A vas promoter drives expression of phiC31\int. The phiC31\int sequence has been engineered to have the codon bias of Drosophila melanogaster. The construct also has vas 3'UTR sequences. (Bischof and Basler, 2007.11.7)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 16 -- 17 Bolwig organ \| presumptive (Horn et al., 2000) larval stage embryonic/larval sense organ (Horn et al., 2000) anal pad (Horn et al., 2000) embryonic/larval central nervous system (Horn et al., 2000) Bolwig organ (Horn et al., 2000) embryonic/larval hindgut (Horn et al., 2000) pupal stage \| late eye (Horn et al., 2000) adult stage eye (Horn et al., 2000) ocellus (Horn et al., 2000)
Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	M{3xP3-RFP.attP} (Bischof and Basler, 2007.11.7) p3xP3-EGFP.vas-int.Dm.attB (Bischof and Basler, 2007.11.7)
descendant(s)
Comments
	Generated by phiC31\int-mediated integration of p3xP3-EGFP.vas-int.Dm.attB into a target "landing site" insertion of the M{3xP3-RFP.attP} construct. (Bischof and Basler, 2007.11.7) Other than the altered integrase coding region, M{vas-int.Dm} is identical to M{vas-int.B}. (Bischof and Basler, 2007.11.7) The resulting inserted construct consists of a P1\loxP site, followed by a Disc\RFP^3xP3.PB marker, followed by another P1\loxP site, followed by a Avic\GFP^E.3xP3 marker, followed by another P1\loxP site, followed by phiC31\int^Dm.vas, which provides the integrase source. The presence of the P1\loxP sites mean that it is possible to eliminate the Disc\RFP^3xP3.PB and Avic\GFP^E.3xP3 markers by cre-mediated recombination. (Bischof and Basler, 2007.11.7)
Stocks Listed in FlyBase ( 46 )
Bloomington	40161 y¹ M{vas-int.Dm}ZH-2A w^* 24483 y¹ M{vas-int.Dm}ZH-2A w^; M{3xP3-RFP.attP}ZH-51D 24480 y¹ M{3xP3-RFP.attP}ZH-2A w^; M{vas-int.Dm}ZH-102D 24481 y¹ M{vas-int.Dm}ZH-2A w^; M{3xP3-RFP.attP'}ZH-22A 24482 y¹ M{vas-int.Dm}ZH-2A w^; M{3xP3-RFP.attP'}ZH-51C 24484 y¹ M{vas-int.Dm}ZH-2A w^; M{3xP3-RFP.attP'}ZH-58A 24485 y¹ M{vas-int.Dm}ZH-2A w^; M{3xP3-RFP.attP'}ZH-68E 24487 y¹ M{vas-int.Dm}ZH-2A w^; M{3xP3-RFP.attP'}ZH-96E 24488 y¹ M{vas-int.Dm}ZH-2A w^; M{3xP3-RFP.attP}ZH-102D 24749 y¹ M{vas-int.Dm}ZH-2A w^*; M{3xP3-RFP.attP}ZH-86Fb
Kyoto	130431 y¹ M{vas-int.Dm}ZH-2A w^; M{3xP3-RFP.attP}ZH-51D 130428 y¹ M{3xP3-RFP.attP}ZH-2A w^; M{vas-int.Dm}ZH-102D
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	3XP3-GFP (Mishra et al., 2010) M{vas-int.Dm} (FlyBase, 1996-, Bischof and Basler, 2007.11.7, Mishra et al., 2010)
Secondary FlyBase IDs

References ( 2 )
Research paper	Mishra et al., 2010, Development 137(17): 2895--2904 Pph13 and Orthodenticle define a dual regulatory pathway for photoreceptor cell morphogenesis and function. [FBrf0211519]
Personal communication to FlyBase	Bischof and Basler, 2007.11.7, Information on M{vas-int.Dm} stocks. Information on M{vas-int.Dm} stocks. [FBrf0200341]