Recombinant construct P{UAS-β-Spec.dsRNA}

General Information
Symbol	P{UAS-β-Spec.dsRNA}	FlyBase ID	FBtp0036975
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Pielage et al., 2006)
Uses	CV term Qualifiers & info Reference binary system (targeted) β-Spec (Pielage et al., 2006) characterization β-Spec (Pielage et al., 2006) RNAi (dsRNA) construct β-Spec (Pielage et al., 2006)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Pielage et al., 2006)
Component Alleles
Allele	β-Spec^{dsRNA.Scer\UAS}
Reference(s)	(Pielage et al., 2006, Pielage et al., 2005, Ramachandran et al., 2009, Koch et al., 2008, Pielage et al., 2008, Bachmann et al., 2010, Mazock et al., 2010)
Molecular data	Construct: Expression of an 544 bp inverted repeat of the β-Spec cDNA, separated by a w intron, is driven by Scer\UAS sequences. The β-Spec sequence corresponds to position 1687 onwards of the cDNA. (Pielage et al., 2005)
Phenotypic class	lethal \| first instar larval stage, with Scer\GAL4^da.G32 (Pielage et al., 2005) lethal \| larval stage, with Scer\GAL4^αTub84B.PL (Mazock et al., 2010) lethal \| third instar larval stage, with Scer\GAL4^sca-537.4 (Pielage et al., 2005) neuroanatomy defective, with Scer\GAL4^elav.PLu (Koch et al., 2008) neuroanatomy defective, with Scer\GAL4^sca-537.4 (Pielage et al., 2005) neurophysiology defective, with Scer\GAL4^sca-537.4 (Pielage et al., 2005)
Phenotype manifest in	bouton, with Scer\GAL4^sca-537.4 (Pielage et al., 2005) embryonic/larval neuromuscular junction, with Scer\GAL4^elav.PLu (Koch et al., 2008) microtubule cytoskeleton & axon, with Scer\GAL4^sca-537.4 (Pielage et al., 2005) NMJ bouton, with Scer\GAL4^elav.PLu (Koch et al., 2008) postsynaptic membrane, with Scer\GAL4^sca-537.4 (Pielage et al., 2005) synapse, with Scer\GAL4^sca-537.4 (Pielage et al., 2005) synaptic vesicle, with Scer\GAL4^sca-537.4 (Pielage et al., 2005)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-β-Spec.dsRNA} (FlyBase, 1992-) UAS-β-Spec^dsRNA (Mazock et al., 2010) UAS-β-spec-RNAi (Bachmann et al., 2010) UAS-β-spectrin (Ramachandran et al., 2009) UAS-β-spectrin^dsRNA (Koch et al., 2008) UAS-β-spectrin-dsRNA (Pielage et al., 2006, Pielage et al., 2008)
Secondary FlyBase IDs

References ( 7 )
Research paper	Bachmann et al., 2010, J. Neurosci. 30(17): 5811--5824 A perisynaptic ménage à trois between Dlg, DLin-7, and Metro controls proper organization of Drosophila synaptic junctions. [FBrf0210640] Mazock et al., 2010, Mol. Biol. Cell 21(16): 2860--2868 Transgene Rescue Identifies an Essential Function for Drosophila {beta} Spectrin in the Nervous System and a Selective Requirement for Ankyrin-2-binding Activity. [FBrf0211527] Ramachandran et al., 2009, Dev. Neurobiol. 69(9): 583--602 A critical step for postsynaptic F-actin organization: Regulation of Baz/Par-3 localization by aPKC and PTEN. [FBrf0208224] Koch et al., 2008, Neuron 58(2): 210--222 Drosophila ankyrin 2 is required for synaptic stability. [FBrf0204749] Pielage et al., 2008, Neuron 58(2): 195--209 A presynaptic giant ankyrin stabilizes the NMJ through regulation of presynaptic microtubules and transsynaptic cell adhesion. [FBrf0204637] Pielage et al., 2006, J. Cell Biol. 175(3): 491--503 A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction. [FBrf0192906] Pielage et al., 2005, Curr. Biol. 15(10): 918--928 Presynaptic spectrin is essential for synapse stabilization. [FBrf0187325]