Recombinant construct P{FRT(Dscam2^{Scer\SceI.RS.cMa})}

General Information
Symbol	P{FRT(Dscam2^{Scer\SceI.RS.cMa})}	FlyBase ID	FBtp0036999
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Millard et al., 2007)
Uses	CV term Qualifiers & info Reference characterization Dscam2 (Millard et al., 2007) FRT construct targeted recombination donor Dscam2 (Millard et al., 2007)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference regulatory/structural DNA (Millard et al., 2007)
Component Alleles
Allele	Dscam2^{Scer\SceI.RS.cMa}
Reference(s)	(Millard et al., 2007)
Molecular data	Contains a w marker gene, immediately flanked upstream and downstream by insulator sequences from pP{Pelican}, followed by 3.5kb and 3.1kb from from upstream and downstream of Dscam2 exon 1. The construct also contains Scer\FRT sites and an Scer\SceI.RS site. (Millard et al., 2007)
Phenotypic class
Phenotype manifest in
Other information

Allele	gypsy\su(Hw)BR⁴³⁰
Reference(s)	(Millard et al., 2007)
Molecular data	Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon. (Kuhn et al., 2003) Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014). (Roseman et al., 1995) Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014. (Roseman et al., 1993) Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites. (Patton et al., 1992)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	Targeting construct for "ends-out" homologous targeting of Dscam2. Contains a w marker gene, immediately flanked upstream and downstream by insulator sequences from pP{Pelican}, followed by 3.5kb and 3.1kb from from upstream and downstream of Dscam2 exon 1. The construct also contains Scer\FRT sites and an Scer\SceI.RS site. (Millard et al., 2007)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{FRT(Dscam2^{Scer\SceI.RS.cMa})} (FlyBase, 1996-) pW37 Dscam2 (Millard et al., 2007)
Secondary FlyBase IDs

References ( 1 )
Supplementary material	Millard et al., 2007, Nature 447(7145): Online Methods. [FBrf0202569]

Recombinant construct P{FRT(Dscam2Scer\SceI.RS.cMa)}

Recombinant construct P{FRT(Dscam2^{Scer\SceI.RS.cMa})}