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General Information
Symbol
PBac{IRES-GFP.GT}
FlyBase ID
FBtp0039294
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Tni\piggyBac
Construct components
Product class / Tool use(s)
Marker(s)
Description

The PBac{IRES-GFP.GT} element contains two trapping cassettes. At one end of the element there is a mutagenic gene trap cassette consisting of an artificial splice acceptor site, three stop signals (one in each frame), an internal ribosome entry site (IRES) and a promoterless EGFP coding sequence. At the other end of the element, a polyA trap cassette is present that consists of the wIRES-GFP.GT minigene with its its own promoter but lacking a 3'-UTR and natural stop signal, followed by a splice donor site. The polyA trap cassette acts as a visible marker for a successful integration into a gene; w is only stably expressed when a poly(A) tail is added by forming a fusion transcript with 3' exon(s) of the trapped host gene.

Component allele wIRES-GFP.GT
Description

A mini-w gene containing its own promoter but lacking a 3'-UTR and natural stop signal, followed by a splice donor site.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
reporter
gene
Component Alleles
Reference(s)
Molecular data

A mini-w gene containing its own promoter but lacking a 3'-UTR and natural stop signal, followed by a splice donor site.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
PBac{IRES-GFP.GT}
Secondary FlyBase IDs
    References (4)