Open Close
General Information
Symbol
P{tub-Rad21.271TEV-myc}
FlyBase ID
FBtp0041242
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
Rad21TEV
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Marker(s)
Product class / Tool use(s)
Description
A tubulin promoter drives expression of vtd, which is tagged 12bp upstream of the translational stop codon with 10 copies of Tag:MYC. In addition, an SpeI site has been introduced into the vtd coding region after amino acid residue 271, and 3 tandem copies of the Tag:CS(TEVp) protein cleavage tag have been introduced into this site (this sequence allows cleavage of the vtd protein by TEV\NIa). The portion of the construct containing the vtd coding region and a 3'UTR sequence is flanked by an FRT cassette.
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
selectable
marker
Component Alleles
Molecular data
A tubulin promoter drives expression of vtd, which is tagged 12bp upstream of the translational stop codon with 10 copies of Tag:MYC. In addition, an SpeI site has been introduced into the vtd coding region after amino acid residue 271, and 3 tandem copies of the Tag:CS(TEVp) protein cleavage tag have been introduced into this site (this sequence allows cleavage of the vtd protein by TEV\NIa). The portion of the construct containing the vtd coding region and a 3'UTR sequence is flanked by an FRT cassette.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
P{tub-Rad21.271TEV-myc}
P{w+, tubpr<Rad21(271-3TEV)-myc10<SV40}
Rad21-(TEV-271)
tub-Rad21(271-3TEV)-myc
Secondary FlyBase IDs
    References (6)