Recombinant construct P{UAS-rtTA-M2-alt}

General Information
Symbol	P{UAS-rtTA-M2-alt}	FlyBase ID	FBtp0041339
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Stebbins et al., 2001)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Stebbins et al., 2001) molecular tag (Stebbins et al., 2001) regulatory/structural DNA (Stebbins et al., 2001) regulatory/structural DNA (Stebbins et al., 2001)
Component Alleles
Allele	scs'^0.5
Reference(s)	(Stebbins et al., 2001)
Molecular data	Construct: 0.5kb of scs' sequences. (Vazquez and Schedl, 1994)
Phenotypic class
Phenotype manifest in
Other information

Allele	scs^fl.cKa
Reference(s)	(Stebbins et al., 2001)
Molecular data	1.8kb BamHI-BglII fragment containing scs sequences. (Sigrist and Pirrotta, 1997) Full length 1.8kb scs insulator. (Cai and Levine, 1995) Construct: 1.8kb of scs sequences. (Vazquez and Schedl, 1994) Full length 1.8kb BamHI-BglII scs insulator. (Kellum and Schedl, 1992)
Phenotypic class
Phenotype manifest in
Other information

Allele	Tn10\tetR^{M2.alt.Scer\UAS.T:Hsim\VP16}
Reference(s)	(Li and Tower, 2009, Stebbins et al., 2001, Ford et al., 2007, Hoe et al., 2011)
Molecular data	Scer\UAS regulatory sequences drive expression of the DNA binding domain of Tn10\tetR fused to the T:Hsim\VP16 transcriptional activation domain. The Tn10\tetR sequences have been mutated to yield greater expression and tighter regulation by doxycyline. (Ford et al., 2007) Scer\UAS regulatory sequences drive expression of "rtTA-M2-alt". (Stebbins et al., 2001)
Phenotypic class	lethal \| tetracycline conditional, with Scer\GAL4^salm-459.2, rpr^{Tn10\tetO.cSa} (Stebbins et al., 2001) long lived, with Scer\GAL4^D42, hebe^{Tn10\tetO.cLa} (Li and Tower, 2009) long lived, with Scer\GAL4^D42, magu^{Tn10\tetO.cLa} (Li and Tower, 2009) long lived \| male limited, with Hsap\UBB^{Tn10\tetO.cHa}, Scer\GAL4^da.G32 (Hoe et al., 2011) short lived \| male limited, with Hsap\UBB^+1.Tn10\tetO, Scer\GAL4^da.G32 (Hoe et al., 2011) visible \| tetracycline conditional, with Scer\GAL4^salm-459.2, rpr^{Tn10\tetO.cSa} (Stebbins et al., 2001)
Phenotype manifest in	eye \| tetracycline conditional, with Scer\GAL4^salm-459.2, rpr^{Tn10\tetO.cSa} (Stebbins et al., 2001) wing \| tetracycline conditional, with Scer\GAL4^salm-459.2, rpr^{Tn10\tetO.cSa} (Stebbins et al., 2001)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-rtTA-M2-alt} (Ford et al., 2007, FlyBase, 1992-) rtTA-M2alt (Hoe et al., 2011) UAS-rtTA (Li and Tower, 2009) UAS-rtTA-M2-alt (Stebbins et al., 2001)
Secondary FlyBase IDs

References ( 4 )
Research paper	Hoe et al., 2011, Aging 3(3): 237--261 Ubiquitin over-expression phenotypes and ubiquitin gene molecular misreading during aging in Drosophila melanogaster. [FBrf0213373] Li and Tower, 2009, Mol. Genet. Genomics 281(2): 147--162 Adult-specific over-expression of the Drosophila genes magu and hebe increases life span and modulates late-age female fecundity. [FBrf0207375] Ford et al., 2007, Exp. Gerontol. 42(6): 483--497 Alteration of Drosophila life span using conditional, tissue-specific expression of transgenes triggered by doxycyline or RU486/Mifepristone. [FBrf0200753]
Supplementary material	Stebbins et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(19): Supporting Information. [FBrf0208316]