Recombinant construct P{KK105437}

General Information
Symbol	P{KK105437}	FlyBase ID	FBtp0044480
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02	New/Merged/Split Record This record was either newly added to FlyBase for this release or is the result of merging or splitting existing records in this release
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Associated Sequence Features	dsRNA-KK105437
Description	CV term Qualifiers & info Reference transposon P-element (Keleman et al., 2009.8.5)
Uses	CV term Qualifiers & info Reference binary system (targeted) GluRIIA (Keleman et al., 2009.8.5) characterization GluRIIA (Keleman et al., 2009.8.5) RNAi (dsRNA) construct GluRIIA (Keleman et al., 2009.8.5)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Keleman et al., 2009.8.5)
Component Alleles
Allele	GluRIIA^KK105437
Reference(s)	(Keleman et al., 2009.8.5, Tsubouchi et al., 2012)
Molecular data	Scer\UAS regulatory sequences drive expression of an inverted repeat. (Keleman et al., 2009.8.5)
Phenotypic class	lethal, with Dcr-2^Scer\UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW (Tsubouchi et al., 2012)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	P{attP,y⁺,w^3'} (Keleman et al., 2009.8.5)
descendant(s)
Comments
	P-element construct created in vivo: the hairpin-containing segment, carried in a pKC26-derived plasmid, was introduced into flies and inserted via site-specific recombination into a genomic copy of P{attP,y[+],w[3']}. (Keleman et al., 2009.8.5)
Stocks Listed in FlyBase ( 1 )
VDRC	v101686 P{KK105437}VIE-260B
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	105437 (Keleman et al., 2009.8.5) P{KK105437} (FlyBase, 1996-, Tsubouchi et al., 2012)
Secondary FlyBase IDs

References ( 2 )
Supplementary material	Tsubouchi et al., 2012, Curr. Biol. 22(22): Tables S1 and S2. RNAi Lines Tested for Screening and Candidate Channel Genes, Related to Figure 5. [FBrf0220657]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]