Recombinant construct P{Prosα6T-EGFP.M}

General Information
Symbol	P{Prosα6T-EGFP.M}	FlyBase ID	FBtp0050456
Feature type	transgenic_transposon

Size		Expression data	GFP reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zhong and Belote, 2007)
Uses	CV term Qualifiers & info Reference characterization Prosα6T (Zhong and Belote, 2007) reporter construct vital \| Prosα6T (Zhong and Belote, 2007)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Zhong and Belote, 2007)
Component Alleles
Allele	Prosα6T^{T:Avic\GFP-EGFP}
Reference(s)	(Zhong and Belote, 2007, Ma et al., 2002, Bader et al., 2010)
Molecular data	Contains the Prosα6T gene, with 0.8kb of upstream sequences and 1.1kb of downstream sequences. The Prosα6T open reading frame is tagged at the C-terminal end with T:Avic\GFP[EGFP]. (Ma et al., 2002)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Ma et al., 2002)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter or direct label Stage Tissue/Position (including subcellular localization) Reference spermatogenesis spermatid (Ma et al., 2002) spermatozoon • sperm flagellum (Ma et al., 2002)
Additional Information
Statement Reference Prosα6T^{T:Avic\GFP-EGFP} is expressed only in the testis, and is not expressed until after the second meiotic division. Expression continues through then end of spermatogenesis. Prosα6T^{T:Avic\GFP-EGFP} can be observed in the tails of mature sperm cells. (Ma et al., 2002)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Alpha6T-GFP (Bader et al., 2010) P{Prosα6T-EGFP.M} (FlyBase, 1992-) Prosα6T-GFP (Zhong and Belote, 2007) pW8/Prosα6T-GFP (Ma et al., 2002)
Secondary FlyBase IDs

References ( 3 )
Research paper	Bader et al., 2010, Development 137(10): 1679--1688 A novel F-box protein is required for caspase activation during cellular remodeling in Drosophila. [FBrf0210629] Zhong and Belote, 2007, Development 134(19): 3517--3525 The testis-specific proteasome subunit Prosalpha6T of D. melanogaster is required for individualization and nuclear maturation during spermatogenesis. [FBrf0201355] Ma et al., 2002, Insect Mol. Biol. 11(6): 627--639 Expression of proteasome subunit isoforms during spermatogenesis in Drosophila melanogaster. [FBrf0152082]