Recombinant construct P{UAS-yki.S168A.GFP.HA}

General Information
Symbol	P{UAS-yki.S168A.GFP.HA}	FlyBase ID	FBtp0051034
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	4 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Oh and Irvine, 2008)
Uses	CV term Qualifiers & info Reference binary system (targeted) yki (Oh and Irvine, 2008) characterization yki (Oh and Irvine, 2008) reporter construct vital \| yki (Oh and Irvine, 2008)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Oh and Irvine, 2008) molecular tag (Oh and Irvine, 2008) molecular tag (Oh and Irvine, 2008) selectable marker (Oh and Irvine, 2008)
Component Alleles
Allele	w^+mC
Reference(s)	(Oh and Irvine, 2008)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}
Reference(s)	(Oh and Irvine, 2008, Varelas et al., 2010, Truscott et al., 2011, Gerhold et al., 2011, Chen and Verheyen, 2012)
Molecular data	Scer\UAS regulatory sequences drive expression of a mutated form of yki which is tagged with T:Avic\GFP[EGFP] and is tagged at the C-terminal end with T:Ivir\HA1. (Oh and Irvine, 2008)
Phenotypic class	increased cell number \| somatic clone, with Scer\GAL4^αTub84B.PL (Gerhold et al., 2011) size defective, with Scer\GAL4^hh-Gal4 (Chen and Verheyen, 2012) size defective, with Scer\GAL4^ptc-559.1 (Chen and Verheyen, 2012) visible, with Scer\GAL4^GMR.PF (Oh and Irvine, 2008) visible, with Scer\GAL4^ptc-559.1 (Chen and Verheyen, 2012)
Phenotype manifest in	eye, with Scer\GAL4^GMR.PF (Oh and Irvine, 2008) eye, with Scer\GAL4^ptc-559.1 (Chen and Verheyen, 2012) haltere, with Scer\GAL4^ptc-559.1 (Chen and Verheyen, 2012) leg, with Scer\GAL4^ptc-559.1 (Chen and Verheyen, 2012) wing disc, with Scer\GAL4^hh-Gal4 (Chen and Verheyen, 2012) wing disc, with Scer\GAL4^ptc-559.1 (Chen and Verheyen, 2012) wing disc \| somatic clone, with Scer\GAL4^Act5C.PI (Oh and Irvine, 2008) wing disc \| somatic clone, with Scer\GAL4^αTub84B.PL (Gerhold et al., 2011)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 2 )
Bloomington	28816 y¹ w^; P{UAS-yki.S168A.GFP.HA}10-7-Y 28836 y¹ w^; P{UAS-yki.S168A.GFP.HA}10-12-1
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-yki.S168A.GFP.HA} (FlyBase, 1996-) P{UAS-yki.S168A.GFP} (FlyBase, 1992-) UAS-yki^S168A (Truscott et al., 2011) UAS-ykiS168A:GFP (Oh and Irvine, 2008) UAS-Yki-S168A:GFP (Varelas et al., 2010) UAS-yorkie^S168A (Gerhold et al., 2011) Yki^S168A (Chen and Verheyen, 2012) Yki-S168A (Varelas et al., 2010) Yki S168A:GFP (Chen and Verheyen, 2012)
Secondary FlyBase IDs

References ( 5 )
Research paper	Chen and Verheyen, 2012, Curr. Biol. 22(17): 1582--1586 Homeodomain-interacting protein kinase regulates yorkie activity to promote tissue growth. [FBrf0219448] Gerhold et al., 2011, Genetics 189(4): 1309--1326 Identification and characterization of genes required for compensatory growth in Drosophila. [FBrf0217000] Truscott et al., 2011, Genes Dev. 25(17): 1820--1834 mir-11 limits the proapoptotic function of its host gene, dE2f1. [FBrf0215211] Varelas et al., 2010, Dev. Cell 18(4): 579--591 The Hippo pathway regulates Wnt/beta-catenin signaling. [FBrf0210631] Oh and Irvine, 2008, Development 135(6): 1081--1088 In vivo regulation of Yorkie phosphorylation and localization. [FBrf0204358]