General Information
Symbol
Mi{MIC}
FlyBase ID
FBtp0051711
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
MiMIC
Size
7.267
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Encoded product / tool
Marker(s)
Description
The Mi{MIC} element contains a mutagenic gene trap cassette that consists of a splice acceptor site, stop codons in all three reading frame, the coding sequence of EGFP and an SV40 polyadenylation signal. A y+mDint2 marker is present downstream of this sequence. The gene trap cassette and the y+mDint2 marker are flanked by inverted attP sites, which allows for the replacement of this entire sequence with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB sites) through recombination-mediated cassette exchange (RMCE) driven by the phiC31:int integrase.
The EGFP sequence present in the Mi{MIC} element does not play a role in its mutagenic activity and is typically not expressed in Mi{MIC} insertions that are in coding introns. However, a cryptic ribosomal initiation site is present before the EGFP sequence, and thus if a Mi{MIC} element is inserted in the 5'UTR of a gene, it can act as a gene trap, resulting in EGFP expression in these cases.
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
(bases )
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
selectable
marker
selectable
marker
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html
Stocks (7,389)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Secondary FlyBase IDs
    References (76)