FB2022_02, released March 29, 2022

Recombinant Construct: Mi{MIC}

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General Information
Symbol
Mi{MIC}
FlyBase ID
FBtp0051711
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
MiMIC
Size
7.267
Expression Data
Associated insertion(s)
7451 available
Molecular map
Description and Uses
Description

The Mi{MIC} element contains a mutagenic gene trap cassette that consists of a splice acceptor site, stop codons in all three reading frame, the coding sequence of EGFP and an SV40 polyadenylation signal. A y+mDint2 marker is present downstream of this sequence. The gene trap cassette and the y+mDint2 marker are flanked by inverted attP sites, which allows for the replacement of this entire sequence with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB sites) through recombination-mediated cassette exchange (RMCE) driven by the phiC31:int integrase.

(Venken et al., 2011)

The EGFP sequence present in the Mi{MIC} element does not play a role in its mutagenic activity and is typically not expressed in Mi{MIC} insertions that are in coding introns. However, a cryptic ribosomal initiation site is present before the EGFP sequence, and thus if a Mi{MIC} element is inserted in the 5'UTR of a gene, it can act as a gene trap, resulting in EGFP expression in these cases.

(Kanca et al., 2017)

A diagram of the Mi{MIC} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

(FlyBase, 1992-)
Construct components
Product class / Tool use(s)
Encoded product / tool
Also carries
attP
Marker(s)
y+mDint2
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
GU370067
(bases )
Segments and Size
Total Size

7.267

(Venken et al., 2009.12.19)
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Mi{2A-lexA::p65.cons}
(Tadros et al., 2016)
Mi{2A-lexA::p65.FRT}
(Tadros et al., 2016)
Mi{3'MIC}
(FlyBase, 1992-)
Mi{attR1-2}
(Kim et al., 2013)
Mi{CadN.AE}
(Pecot et al., 2013)
Mi{Fas2.PC-V5,PB-HA}
(Neuert et al., 2020)
Mi{GT-FLPo}
(Venken et al., 2011)
Mi{GT-GAL4}
(Venken et al., 2011)
Mi{GT-GAL80}
(Luo, 2018.1.22)
Mi{GT-mCh}
(Venken et al., 2011)
Mi{GT-QF2}
(Luo, 2018.1.22)
Mi{GT-QF}
(Venken et al., 2011)
Mi{PlexB.CT}
(Li et al., 2018)
Mi{PT-BM.0}
(Venken et al., 2011)
Mi{PT-BM.1}
(Venken et al., 2011)
Mi{PT-BM.2}
(Venken et al., 2011)
Mi{PT-BM.un}
(FlyBase, 1992-)
Mi{PT-DV.0}
(Venken et al., 2011)
Mi{PT-DV.1}
(Venken et al., 2011)
Mi{PT-DV.2}
(Venken et al., 2011)
Mi{PT-DV.un}
(FlyBase, 1992-)
Mi{PT-EGFP-V5.un}
(Ramdas Nair et al., 2016)
Mi{PT-GFSTF.0}
(Venken et al., 2011)
Mi{PT-GFSTF.1}
(Venken et al., 2011)
Mi{PT-GFSTF.2}
(Venken et al., 2011)
Mi{PT-GFSTF.un}
(FlyBase, 1992-)
Mi{PT-HS.0}
(Venken et al., 2011)
Mi{PT-HS.1}
(Venken et al., 2011)
Mi{PT-HS.2}
(Venken et al., 2011)
Mi{PT-HS.un}
(FlyBase, 1992-)
Mi{PT-KV.0}
(Venken et al., 2011)
Mi{PT-KV.1}
(Venken et al., 2011)
Mi{PT-KV.2}
(Venken et al., 2011)
Mi{PT-KV.un}
(FlyBase, 1992-)
Mi{PT-mCh.0}
(Venken et al., 2011)
Mi{PT-mCh.1}
(Venken et al., 2011)
Mi{PT-mCh.2}
(Venken et al., 2011)
Mi{PT-mCh.un}
(FlyBase, 1992-)
Mi{PT-tdEGFP.un}
(Kamiyama et al., 2015)
Mi{PT-TRH.0}
(Venken et al., 2011)
Mi{PT-TRH.1}
(Venken et al., 2011)
Mi{PT-TRH.2}
(Venken et al., 2011)
Mi{PT-TRH.un}
(FlyBase, 1992-)
Mi{Sema1a.AE}
(Pecot et al., 2013)
Mi{Trojan-3XGAL80.0}
(Diao et al., 2015)
Mi{Trojan-3XGAL80.1}
(Diao et al., 2015)
Mi{Trojan-3XGAL80.2}
(Diao et al., 2015)
Mi{Trojan-3XGAL80.A}
(Diao et al., 2016)
Mi{Trojan-dVP16AD.0}
(Diao et al., 2015)
Mi{Trojan-dVP16AD.1}
(Diao et al., 2015)
Mi{Trojan-dVP16AD.2}
(Diao et al., 2015)
Mi{Trojan-GAL4.0}
(Diao et al., 2015)
Mi{Trojan-GAL4.1}
(Diao et al., 2015)
Mi{Trojan-GAL4.2.A}
(Diao et al., 2016)
Mi{Trojan-GAL4.2.B}
(Diao et al., 2016)
Mi{Trojan-GAL4.2}
(Diao et al., 2015)
Mi{Trojan-GAL4.no-pA.0}
(Diao et al., 2015)
Mi{Trojan-GAL4.no-pA.1}
(Diao et al., 2015)
Mi{Trojan-GAL4.no-pA.2}
(Diao et al., 2015)
Mi{Trojan-GAL4DBD.0}
(Diao et al., 2015)
Mi{Trojan-GAL4DBD.1}
(Diao et al., 2015)
Mi{Trojan-GAL4DBD.2}
(Diao et al., 2015)
Mi{Trojan-lexA:QFAD.0}
(Diao et al., 2015)
Mi{Trojan-lexA:QFAD.1}
(Diao et al., 2015)
Mi{Trojan-lexA:QFAD.2}
(Diao et al., 2015)
Mi{Trojan-p65AD.0}
(Diao et al., 2015)
Mi{Trojan-p65AD.1}
(Diao et al., 2015)
Mi{Trojan-p65AD.2.A}
(Diao et al., 2016)
Mi{Trojan-p65AD.2.B}
(Diao et al., 2017)
Mi{Trojan-p65AD.2}
(Diao et al., 2015)
Mi{Trojan-QF2.0}
(Diao et al., 2015)
Mi{Trojan-QF2.1}
(Diao et al., 2015)
Mi{Trojan-QF2.2.B}
(Diao et al., 2016)
Mi{Trojan-QF2.2}
(Diao et al., 2015)
Mi{UASp-EGFP.DL}
(DeLuca and Spradling, 2018)
Mi{UASt-EGFP.DL}
(DeLuca and Spradling, 2018)
Comments
Stocks (7,344)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
MIMIC
(Venken and Levis, 2010.4.2, Venken et al., 2009.12.19)
MiMIC
(Nagarkar-Jaiswal et al., 2015, Venken et al., 2011)
Mi{MIC}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (83)