Recombinant construct P{FRT(Δ96TG.GFP⁺).w⁺}

General Information
Symbol	P{FRT(Δ96TG.GFP⁺).w⁺}	FlyBase ID	FBtp0051882
Feature type	transgenic_transposon

Size		Expression data	GFP reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (King-Jones et al., 2006)
Uses	CV term Qualifiers & info Reference characterization Hr96 (King-Jones et al., 2006) FRT construct targeted recombination donor Hr96 (King-Jones et al., 2006)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (King-Jones et al., 2006) selectable marker (King-Jones et al., 2006)
Component Alleles
Allele	Avic\GFP^E.3xP3
Reference(s)	(King-Jones et al., 2006)
Molecular data	Three tandem repeats of an artificially derived Mmus\Pax6 homodimer binding site are fused upstream of a TATA box driving expression of "EGFP". (Horn et al., 2000) Construct: Artificial promoter containing three binding sites for Mmus\Pax6 homodimers in front of a TATA box drives the expression of the enhanced variant of Avic\GFP (EGFP). (Berghammer et al., 1999)
Phenotypic class
Phenotype manifest in
Other information

Allele	Hr96^{Δ96TG.GFP.Scer\SceI.RS}
Reference(s)	(King-Jones et al., 2006)
Molecular data	A 7.55kb genomic fragment that contains the entire Hr96 gene along with two engineered deletions in the protein coding sequences. The first deletion is 26bp in length and removes the start codon. The second deletion is 331bp in length, removing all of exon 4, the downstream intron and the splice acceptor site for exon 5. A Scer\SceI.RS site has been inserted into the middle of the 7.55kb fragment (to make a cut between nucleotides 3699 and 3700). (King-Jones et al., 2006)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(King-Jones et al., 2006)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 16 -- 17 Bolwig organ \| presumptive (Horn et al., 2000) larval stage embryonic/larval sense organ (Horn et al., 2000) anal pad (Horn et al., 2000) embryonic/larval central nervous system (Horn et al., 2000) Bolwig organ (Horn et al., 2000) embryonic/larval hindgut (Horn et al., 2000) pupal stage \| late eye (Horn et al., 2000) adult stage eye (Horn et al., 2000) ocellus (Horn et al., 2000)
Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	Donor construct for ends-in targeting of Hr96. (King-Jones et al., 2006) Contains a 7.55kb genomic fragment that contains the entire Hr96 gene along with two engineered deletions in the protein coding sequences. The first deletion is 26bp in length and removes the start codon. The second deletion is 331bp in length, removing all of exon 4, the downstream intron and the splice acceptor site for exon 5. A Scer\SceI.RS site has been inserted into the middle of the 7.55kb fragment (to make a cut between nucleotides 3699 and 3700). Also contains a Avic\GFP[E.3xP3] marker. A Crei\I-CreI.RS site is present 5' to the Avic\GFP[E.3xP3] marker. The Hr96 and Avic\GFP[E.3xP3] marker sequences are contained within an Scer\FRT cassette. (King-Jones et al., 2006)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	<up><Δ96TG GFP⁺>^w+</up> (King-Jones et al., 2006) P{FRT(Δ96TG.GFP⁺).w⁺} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Supplementary material	King-Jones et al., 2006, Cell Metab. 4(1): Supplemental data. [FBrf0209926]

Recombinant construct P{FRT(Δ96TG.GFP+).w+}

Recombinant construct P{FRT(Δ96TG.GFP⁺).w⁺}