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General Information
Symbol
P{fwe-8.2g-YFP-GFP-RFP}
FlyBase ID
FBtp0052135
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
Construct components
Product class / Tool use(s)
Regulatory region(s)
Description

An 8.2kb fragment covering the fwe gene locus, modified such that YFP is inserted downstream of the -RA transcript, GFP is tagged onto the C-terminal of -RB and mRFP(Unk) is inserted downstream of the -RC isoform.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
molecular
tag
Component Alleles
Molecular data

An 8.2kb fragment covering the fwe gene locus, modified such that YFP is inserted downstream of the -RA transcript, GFP is tagged onto the C-terminal of -RB and mRFP(Unk) is inserted downstream of the -RC isoform.

Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

fwe+8.2.RA.YFP.RB.GFP.RC.mRFP(Unk) generates differentially tagged isoforms of fwe, allowing isoform-specific expression pattern analysis. GFP-tagged fwe-PC (fweLose-A) is expressed in all retinal neurons from 24-46 hours APF. RFP-tagged fwe-PB (fweLoseB) is not expressed until 36 hours APF, in ommatidia at the periphery of the retina, where photoreceptor neurons are undergoing programmed cell death; this expression is maintained until 44 hours APF.

 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
Reported As
Symbol Synonym
Flower reporter
FweLose-A-GFP
FweLose-B-RFP
P{flower-8.2g-YFP-HA-MYC}
P{fwe-8.2g-YFP-GFP-RFP}
flowerLoseA-GFP
flowerLoseB-RFP
flowerUbi-YFPflowerLose-A-GFP flowerLose-B-RFP
flowerubi-YFP
flowerUbi-YFP,flowerLose-A-GFP,flowerLose-B-RFP
Secondary FlyBase IDs
    References (7)