ScerGAL4lig.Elig is expressed in most of the glial cells in the brain of late third instar larva, including subperineurial, neuropil-associated and cortex glia. ScerGAL4lig.Elig is also expressed in a cluster of neurons in the dorsomedial brain that project contralaterally. In the ventral nerve cord, expression is found in surface glia cells including subperineurial and exit glial cells. ScerGAL4lig.Elig was also observed in many neurons of the ventral nerve cord, which are not motorneurons.