Recombinant construct P{UAS-dlp.-HS}

General Information
Symbol	P{UAS-dlp.-HS}	FlyBase ID	FBtp0053984
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Yan et al., 2009)
Uses	CV term Qualifiers & info Reference binary system (targeted) dlp (Yan et al., 2009) characterization dlp (Yan et al., 2009)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Yan et al., 2009) selectable marker (Yan et al., 2009)
Component Alleles
Allele	dlp^-HS.Scer\UAS
Reference(s)	(Yan et al., 2009, Yan et al., 2009, Yan et al., 2010)
Molecular data	Scer\UAS regulatory sequences drive expression of a mutated form of dlp in which all five potential GAG attachment sites (Ser625, Ser629, Ser631, Ser643 and Ser686) have been converted to Ala. (Yan et al., 2009) Scer\UAS regulatory sequences drive expression of a mutated form of dlp in which all of the GAG attachment sites have been mutated and also the GPI anchor has been replaced by the T:Rnor\CD2 transmembrane protein. (Yan et al., 2009)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Yan et al., 2009)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-dlp.-HS} (FlyBase, 1992-) UAS-dlp(-HS) (Yan et al., 2009, Yan et al., 2009, Yan et al., 2010)
Secondary FlyBase IDs

References ( 3 )
Research paper	Yan et al., 2010, Development 137(12): 2033--2044 The cell-surface proteins Dally-like and Ihog differentially regulate Hedgehog signaling strength and range during development. [FBrf0210906] Yan et al., 2009, Dev. Cell 17(4): 470--481 The core protein of glypican Dally-like determines its biphasic activity in wingless morphogen signaling. [FBrf0209080]
Supplementary material	Yan et al., 2009, Dev. Cell 17(4): Supplemental Data. [FBrf0211306]