Recombinant construct P{Gr28b.d-GAL4}

General Information
Symbol	P{Gr28b.d-GAL4}	FlyBase ID	FBtp0054529
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Thorne and Amrein, 2008)
Uses	CV term Qualifiers & info Reference binary system (regulatory) Gr28b (Thorne and Amrein, 2008)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Thorne and Amrein, 2008)
Component Alleles
Allele	Scer\GAL4^Gr28b.d.PT
Reference(s)	(Thorne and Amrein, 2008, Weiss et al., 2011, Weiss et al., 2011, Silbering et al., 2011, Kwon et al., 2011)
Molecular data	A 772bp fragment containing the promoter region immediately upstream of the d-isoform of Gr28b is cloned upstream of Scer\GAL4 coding sequences. (Thorne and Amrein, 2008)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference adult stage gustatory receptor neuron \| subset (Thorne and Amrein, 2008) gustatory receptor neuron of labellar taste bristle \| subset (Thorne and Amrein, 2008) gustatory receptor neuron \| subset of ventral cibarial sense organ (Thorne and Amrein, 2008) gustatory receptor neuron \| faint & subset of prothoracic tarsal segment 5 (Thorne and Amrein, 2008) neuron \| faint & subset of prothoracic tarsal segment 5 (Thorne and Amrein, 2008) neuron \| subset of arista (Thorne and Amrein, 2008) sensillum campaniformium \| subset of wing vein L3 (Thorne and Amrein, 2008) sensillum campaniformium \| subset of wing vein L1 (Thorne and Amrein, 2008) mechanosensory neuron \| subset of leg (Thorne and Amrein, 2008) prothoracic femoral chordotonal organ (Thorne and Amrein, 2008) metathoracic femoral chordotonal organ (Thorne and Amrein, 2008) mesothoracic femoral chordotonal organ (Thorne and Amrein, 2008) dendritic arborizing neuron \| subset of abdomen \| dorsal (Thorne and Amrein, 2008) gustatory receptor neuron \| subset of short labellar taste bristle \| subset (Weiss et al., 2011) L2 GRN of labellum sensillum (Weiss et al., 2011) S-type sensilla S5 (Weiss et al., 2011, Weiss et al., 2011) Comment:isoform Gr28b-RD adult antennal lobe glomerulus (Thorne and Amrein, 2008)
Additional Information
Statement Reference Expression of Scer\GAL4[Gr28b.d.PT] is seen in approximately 3 neurons per labial palp. Scer\GAL4[Gr28b.d.PT] is found to be expressed in a single neuron associated with the ventral cibarial sense organ. Expression is found in at least two taste neurons and also in several non-chemosensory neurons associated with the fifth tarsal segment. Expression of Scer\GAL4[Gr28b.d.PT] is seen in three neurons located at the base of the arista, which possibly function as thermo- and/or hygroreceptors. Expression is seen in leg neurons thought to be stretch receptors or proprioreceptors. Expression of Scer\GAL4[Gr28b.d.PT] is seen within axons that innervate either antennal lobe glomerulus VP2 or VP3. (Thorne and Amrein, 2008) Scer\GAL4[Gr28b.d.PT] is expressed in one short bitter-sensitive sensilla of the labellum. (Weiss et al., 2011) Scer\GAL4[Gr28b.d.PT] is expressed in a gustatory neuron of one bitter-sensitive labellar taste bristle. (Weiss et al., 2011) Scer\GAL4[Gr28b.d.PT] is expressed in one short bitter-sensitive sensilla of the labellum. (Weiss et al., 2011)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	GR28b.d-GAL4 (Silbering et al., 2011) Gr28b.d-Gal4 (Thorne and Amrein, 2008) Gr28b.d-GAL4 (Weiss et al., 2011, Kwon et al., 2011) P{Gr28b.d-GAL4} (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 5 )
Research paper	Kwon et al., 2011, J. Neurosci. 31(43): 15300--15309 Molecular and cellular organization of the taste system in the Drosophila larva. [FBrf0216529] Silbering et al., 2011, J. Neurosci. 31(38): 13357--13375 Complementary Function and Integrated Wiring of the Evolutionarily Distinct Drosophila Olfactory Subsystems. [FBrf0215822] Weiss et al., 2011, Neuron 69(2): 258--272 The Molecular and Cellular Basis of Bitter Taste in Drosophila. [FBrf0212834] Thorne and Amrein, 2008, J. Comp. Neurol. 506(4): 548--568 Atypical expression of Drosophila gustatory receptor genes in sensory and central neurons. [FBrf0201488]
Supplementary material	Weiss et al., 2011, Neuron 69(2): Supplemental Information. [FBrf0213039]