A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Recombinant construct P{FRT(mre11.Scer\SceI.RS.armGFP)}

General Information
Symbol P{FRT(mre11.Scer\SceI.RS.armGFP)} FlyBase ID FBtp0055377
Feature type transgenic_transposon
Size Expression data
Associated insertions 0 available
Molecular map
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Description
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FB2013_03
FB2013_02
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hide Description & Uses
Species synthetic construct
Location-dependent
role
Description
CV term
Qualifiers & info
Reference
transposon
P-element
Uses
CV term
Qualifiers & info
Reference
characterization
FRT construct
reporter construct
vital | arm
targeted recombination donor
Cloning Sites
Location
Restriction sites
Reference
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Sequence (FB)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
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Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
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CV term
Qualifiers & info
Reference
reporter
hide Component Alleles
Allele Avic\GFParm.PG
Reference(s) (Gong et al., 2005)
Molecular data
A 1.8kb arm promoter fragment drives expression of the EGFP variant of Avic\GFP.
Phenotypic class
Phenotype manifest in
Other information
Allele mre11Scer\SceI.RS.cGa
Reference(s) (Gong et al., 2005)
Molecular data
Contains two fragments that normally flank the mre11 gene. The upstream homologous fragment is a 2.5kb fragment from immediately upstream of the mre11 start codon. This is followed by the Avic\GFP[arm.PG] marker. The downstream homologous fragment is a 2.9kb fragment from downstream of the mre11 gene. A Scer\SceI.RS restriction site is present upstream of the upstream homologous fragment.
Phenotypic class
Phenotype manifest in
Other information
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Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects expression of
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progenitor(s)
descendant(s)
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Targeting construct for ends-out homologous targeting of mre11. Contains two fragments that normally flank the mre11 gene. The upstream homologous fragment is a 2.5kb fragment from immediately upstream of the mre11 start codon. This is followed by the Avic\GFP[arm.PG] marker. The downstream homologous fragment is a 2.9kb fragment from downstream of the mre11 gene. A Scer\SceI.RS restriction site is present upstream of the upstream homologous fragment.
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Other Crossreferences
Linkouts
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Reported As
Symbol Synonym
P{FRT(mre11.Scer\SceI.RS.armGFP)}
Secondary FlyBase IDs
hide References ( 1 )
Research paper
Gong et al., 2005, D. I. S. 88: 79--83
Targeted mutagenesis of Drosophila atm and mre11 genes. [FBrf0211655]