Recombinant construct P{Trh-GAL4.long}

General Information
Symbol	P{Trh-GAL4.long}	FlyBase ID	FBtp0055412
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Alekseyenko et al., 2010)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Alekseyenko et al., 2010)
Component Alleles
Allele	Scer\GAL4^l.Trh.PK
Reference(s)	(Alekseyenko et al., 2010, Chiang et al., 2011, Luo et al., 2012, Neckameyer and Bhatt, 2012, Liu et al., 2012)
Molecular data	A 1.7kb fragment of Trh regulatory sequences is cloned upstream of Scer\GAL4 coding sequences. (Alekseyenko et al., 2010)
Phenotypic class	behavior defective \| heat sensitive, with shi^1.Scer\UAS (Alekseyenko et al., 2010) behavior defective \| heat sensitive, with TrpA1^Scer\UAS.cKa (Alekseyenko et al., 2010) feeding behavior defective, with D2R^JF02025 (Neckameyer and Bhatt, 2012) feeding behavior defective, with Trh^{dsRNA.Sym.Scer\UAS} (Neckameyer and Bhatt, 2012)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference adult stage LP2 neuron (Alekseyenko et al., 2010) serotonergic SE1 neuron (Alekseyenko et al., 2010) FMRFamide SE2 neuron (Alekseyenko et al., 2010) serotonergic SE3 neuron (Alekseyenko et al., 2010) adult protocerebrum \| restricted (Alekseyenko et al., 2010) posterior lateral protocerebrum \| restricted (Alekseyenko et al., 2010)
Additional Information
Statement Reference Scer\GAL4[l.Trh.PK] is seen to be expressed in the posterior medial protocerebrum (PMP), the AMP (anterior medial protocerebrum) and the ALP (anterior lateral protocerebrum). (Alekseyenko et al., 2010)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 2 )
Bloomington	38388 w¹¹¹⁸; P{Trh-GAL4.long}2 38389 w¹¹¹⁸; P{Trh-GAL4.long}3
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	DTRHGal4 (Neckameyer and Bhatt, 2012) P{Trh-GAL4.long} (FlyBase, 1992-) Trh-GAL4 (Chiang et al., 2011) Trh-Gal4 (Chiang et al., 2011) TRH-Gal4 (Liu et al., 2012, Luo et al., 2012)
Secondary FlyBase IDs

References ( 6 )
Research paper	Liu et al., 2012, Curr. Biol. 22(22): 2114--2123 Two dopaminergic neurons signal to the dorsal fan-shaped body to promote wakefulness in Drosophila. [FBrf0220041] Luo et al., 2012, Cell. Molec. Life Sci. 69(3): 471--484 Insulin-producing cells in the brain of adult Drosophila are regulated by the serotonin 5-HT(1A) receptor. [FBrf0217219] Neckameyer and Bhatt, 2012, BMC Neurosci. 13: 26 Neurotrophic actions of dopamine on the development of a serotonergic feeding circuit in Drosophila melanogaster. [FBrf0218484] Chiang et al., 2011, Curr. Biol. 21(1): 1--11 Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution. [FBrf0212704] Alekseyenko et al., 2010, PLoS ONE 5(5): e10806 Targeted manipulation of serotonergic neurotransmission affects the escalation of aggression in adult male Drosophila melanogaster. [FBrf0210961]
Personal communication to FlyBase	Alekseyenko and Kravitz, 2013.1.21, P{Trh-GAL4.long}2 & P{Trh-GAL4.long}3. P{Trh-GAL4.long}2 & P{Trh-GAL4.long}3. [FBrf0220593]