Open Close
General Information
Symbol
P{UAS-Apoliner}
FlyBase ID
FBtp0055503
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
UAS-Apoliner
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele Diap1Apoliner.UAS
Product class / Tool use(s)
Description

UASt regulatory sequences drive expression of 'Apoliner', a fluorescent sensor for caspase activity. The sensor is comprised of two fluorescent proteins (mRFP1 and EGFP) separated by sequence derived from the Diap1 gene. The Diap1 sequence corresponds to amino acids 1-146 and includes a caspase cleavage site, DQVDGV (this site has been mutated from the original DQVDNN to prevent degradation of the cleaved product by the N-end rule pathway). In addition, a transmembrane domain (Tag:M(mCd8a)) is fused to the N-terminal end of the mRFP1 sequence and a nuclear localization signal (Tag:NLS(SV40-largeT)) is fused to the N-terminal end of the EGFP sequence. In the absence of caspase activity, the transmembrane domain tethers both fluorescent proteins to the membrane. Upon cleavage of the DQVDGV site by active caspase, the EGFP protein is released, and can be targeted to the nucleus via the Tag:NLS(SV40-largeT) signal, while the mRFP1 protein remains tethered at the membrane.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
Component Alleles
Molecular data

UASt regulatory sequences drive expression of 'Apoliner', a fluorescent sensor for caspase activity. The sensor is comprised of two fluorescent proteins (mRFP1 and EGFP) separated by sequence derived from the Diap1 gene. The Diap1 sequence corresponds to amino acids 1-146 and includes a caspase cleavage site, DQVDGV (this site has been mutated from the original DQVDNN to prevent degradation of the cleaved product by the N-end rule pathway). In addition, a transmembrane domain (Tag:M(mCd8a)) is fused to the N-terminal end of the mRFP1 sequence and a nuclear localization signal (Tag:NLS(SV40-largeT)) is fused to the N-terminal end of the EGFP sequence. In the absence of caspase activity, the transmembrane domain tethers both fluorescent proteins to the membrane. Upon cleavage of the DQVDGV site by active caspase, the EGFP protein is released, and can be targeted to the nucleus via the Tag:NLS(SV40-largeT) signal, while the mRFP1 protein remains tethered at the membrane.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (3)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (12)