Recombinant construct P{UAS-GCaMP3.T}

General Information
Symbol	P{UAS-GCaMP3.T}	FlyBase ID	FBtp0055575
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Tian et al., 2009)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric protein (Tian et al., 2009)
Component Alleles
Allele	Avic\GFP^{GCaMP3.Scer\UAS}
Reference(s)	(Inagaki et al., 2012, Yuan et al., 2011, Tian et al., 2009, Cheng et al., 2010, Hatan et al., 2011, Danjo et al., 2011, Séjourné et al., 2011, Sudhakaran et al., 2012, Liu et al., 2010, Gong et al., 2010, Tsubouchi et al., 2012)
Molecular data	Scer\UAS regulatory sequences drive expression of "GCaMP3", an improved version of "GCaMP". GCaMP3 consists of the M13 fragment of T:Ggal\MLCK, a circularly permuted fluorescent protein (EGFP residues 149-238 containing the mutations M153K and T203V, followed by EGFP residues 1-144) and the calcium binding domain of T:Rat\Cam (residues 2-148, with the mutation N60D). (Tian et al., 2009)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	32116 w^*; P{UAS-GCaMP3.T}attP40
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	GCaMP3 (Tian et al., 2009, Yuan et al., 2011) GCAMP3 (Sudhakaran et al., 2012) GCAMP3.0 (Gong et al., 2010) P{UAS-GCaMP3.T} (FlyBase, 1992-) UAS-GCaMP3 (Cheng et al., 2010, Séjourné et al., 2011, Hatan et al., 2011, Danjo et al., 2011) UAS-GCaMP3.0 (Tsubouchi et al., 2012, Inagaki et al., 2012) UAS-GCAMP3.0 (Liu et al., 2010)
Secondary FlyBase IDs

References ( 11 )
Research paper	Inagaki et al., 2012, Cell 148(3): 583--595 Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing. [FBrf0217362] Sudhakaran et al., 2012, J. Neurosci. 32(21): 7225--7231 Plasticity of recurrent inhibition in the Drosophila antennal lobe. [FBrf0218409] Tsubouchi et al., 2012, Curr. Biol. 22(22): 2124--2134 Dendritic Filopodia, Ripped Pocket, NOMPC, and NMDARs Contribute to the Sense of Touch in Drosophila Larvae. [FBrf0219946] Danjo et al., 2011, PLoS ONE 6(2): e17131 A tripartite synapse model in Drosophila. [FBrf0213114] Hatan et al., 2011, J. Cell Biol. 192(2): 307--319 The Drosophila blood brain barrier is maintained by GPCR-dependent dynamic actin structures. [FBrf0212807] Séjourné et al., 2011, Nat. Neurosci. 14(7): 903--910 Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila. [FBrf0214029] Yuan et al., 2011, Science 333(6048): 1458--1462 Light-induced structural and functional plasticity in Drosophila larval visual system. [FBrf0215276] Cheng et al., 2010, Neuron 67(3): 373--380 The Role of the TRP Channel NompC in Drosophila Larval and Adult Locomotion. [FBrf0211468] Gong et al., 2010, Science 330(6003): 499--502 Two pairs of neurons in the central brain control Drosophila innate light preference. [FBrf0212127] Tian et al., 2009, Nat. Methods 6(12): 875--881 Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. [FBrf0209330]
Supplementary material	Liu et al., 2010, Science 330(6003): Supporting Online Material: Materials and methods. [FBrf0220458]