FB2025_01 , released February 20, 2025
Recombinant Construct: P{UASp-Venus-solo}
Open Close
General Information
Symbol
P{UASp-Venus-solo}
FlyBase ID
FBtp0055595
Feature type
transgenic_transposable_element
Component Allele(s)
soloN.UASp.Venus
Also Known As
UASp-Venus::SOLO
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele soloN.UASp.Venus
Transgenic product class
wild_type
(Yan et al., 2010)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
solo
Tagged with
Venus
Description

UASp regulatory sequences drive expression of the solo coding region and 3' untranslated region. The protein is tagged at the N-terminal end with Venus.

(Yan et al., 2010)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male
spermatogonium
(Yan et al., 2010)
spermatocyte

Comment: early prophase I to metaphase II

(Yan et al., 2010)
adult stage | female
germarium
(Yan et al., 2010)
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
germarium | posterior
(Yan and McKee, 2013)
oocyte
(Yan and McKee, 2013)
nurse cell
(Yan and McKee, 2013)
presumptive oocyte
(Yan and McKee, 2013)
presumptive nurse cell
(Yan and McKee, 2013)
Additional Information
Statement
Reference

solo is expressed in mitotic cells in the posterior region of the germarium, but not in the anterior-most region of the germarium that contains stem cells and early cystoblasts.

(Yan and McKee, 2013)

solo foci were seen in nuclei of spermatocytes of all stages up to and including metaphase II but were absent at anaphase II and subsequent stages.

(Yan et al., 2010)
 
Marker for
Reflects expression of
solo
(Yan and McKee, 2013, Yan et al., 2010)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{UASp-Venus-solo}
(FlyBase, 1992-)
UAS-SOLO
(Yan and McKee, 2013)
UASp-Venus::SOLO
(Yan and McKee, 2013, Yan et al., 2010)
Venus::SOLO
(Krishnan et al., 2014)
Secondary FlyBase IDs
    References (4)