FB2025_01 , released February 20, 2025
Recombinant Construct: P{Mt2.genTG.EGFP}
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General Information
Symbol
P{Mt2.genTG.EGFP}
FlyBase ID
FBtp0055856
Feature type
transgenic_transposable_element
Component Allele(s)
Mt2genTG.EGFP
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Mt2genTG.EGFP
Transgenic product class
genomic_DNA
(Schaefer et al., 2008)
wild_type
(Schaefer et al., 2008)
Component
Use(s)
Regulatory region(s)
Mt2
Encoded product / tool
Mt2
Tagged with
EGFP
Description

Genomic fragment that encompasses 300bp upstream of the Mt2 start site and 150bp downstream of the stop codon.

(Schaefer et al., 2008)

Genomic fragment containing Mt2 which has been tagged with EGFP.

(Schaefer et al., 2010)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 17
organism | ubiquitous
(Schaefer et al., 2008)
embryonic stage 2 -- 6
cell

Comment: interphase

(Schaefer et al., 2008)
cell

Comment: mitosis

(Schaefer et al., 2008)
third instar larval stage
embryonic/larval salivary gland
(Schaefer et al., 2008)
adult stage | female
ovary
(Schaefer et al., 2008)
ovary
• nucleus
(Schaefer et al., 2008)
adult stage | male
testis

Comment: NOT hub cell of testis, NOT spermatozoon

(Schaefer et al., 2008)
testis
• nucleus

Comment: NOT hub cell of testis, NOT spermatozoon

(Schaefer et al., 2008)
adult stage | female & oogenesis
ovary
(Schaefer et al., 2010)
Additional Information
Statement
Reference

The Mt2genTG.T:AvicGFP-EGFP protein is enriched in the cytoplasm of ovaries in a homogenous distribution. Its distribution changes to a granular pattern upon heat shock.

(Schaefer et al., 2010)

The Mt2genTG.T:AvicGFP-EGFP protein is ubiquitously expressed throughout development, including most stages of gametogenesis, but not in mature sperm or the hub cell of the testis.

(Schaefer et al., 2008)

At the subcellular level, the Mt2genTG.T:AvicGFP-EGFP protein is mostly cytoplasmic, with some present in the nucleus. In early embryos, the cytoplasmic enrichment of Mt2genTG.T:AvicGFP-EGFP occurs at interphase, but the protein redistributes around nuclear structures during mitosis.

(Schaefer et al., 2008)
 
Marker for
Reflects expression of
Mt2
(Schaefer et al., 2010, Schaefer et al., 2008)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
D2-EGFP
(Durdevic et al., 2013)
D2-TG
(Durdevic et al., 2013)
Dnmt-EGFP
(Durdevic et al., 2013)
Dnmt2-EGFP
(Schaefer et al., 2008)
P{Mt2.genTG.EGFP}
(FlyBase, 1992-)
pGeno-Dnmt2-EGFP
(Schaefer et al., 2008)
Secondary FlyBase IDs
    References (4)