FlyBase Recombinant Construct Report: P{Gr64f-GAL4.9.7}

General Information

Symbol

P{Gr64f-GAL4.9.7}

FlyBase ID

FBtp0057275

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^Gr64f.9.7

Also Known As

Gr64f-GAL4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

2 available

Molecular map

Description and Uses

Location-dependent role

Description

CV term

Qualifiers and info

Reference

transposon

P-element

(Weiss et al., 2011)

Construct components

Component allele Scer\GAL4^Gr64f.9.7

Product class / Tool use(s)

Regulatory region(s)

Gr64f

Encoded product / tool

GAL4

binary expression system - driver

Description

Approximately 10kb of Gr64f promoter sequence is fused upstream of sequence encoding a GAL4 driver.

(Dahanukar et al., 2007)

A 9.7kb Gr64f promoter fragment is fused upstream of sequence encoding a GAL4 driver.

(Weiss et al., 2011)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Features

CV term

Qualifiers and info

Reference

binary

component

(Weiss et al., 2011)

Component Alleles

Allele

Scer\GAL4^Gr64f.9.7

Reference(s)

(Li and Montell, 2021, Li et al., 2020, May et al., 2020, McKellar et al., 2020, Vaziri et al., 2020, Chen et al., 2019, May et al., 2019, Musso et al., 2019, Stern et al., 2019, Wu et al., 2019, Youn et al., 2018, Ahn et al., 2017, Chen and Amrein, 2017, Delventhal et al., 2017, Ganguly et al., 2017, Joseph et al., 2017, Kim et al., 2017, Mohammad et al., 2017, Sánchez-Alcañiz et al., 2017, Sung et al., 2017, Talay et al., 2017, Jeong et al., 2016, Jourjine et al., 2016, Thoma et al., 2016, Wang et al., 2016, Yapici et al., 2016, Zhan et al., 2016, Fujii et al., 2015, Shankar et al., 2015, Shim et al., 2015, Klapoetke et al., 2014, Koh et al., 2014, Kwon et al., 2014, Ling et al., 2014, Sun et al., 2014, Yavuz et al., 2014, Fan et al., 2013, Fan et al., 2013, Thistle et al., 2012, Kwon et al., 2011, Park and Kwon, 2011, Weiss et al., 2011, Weiss et al., 2011, Dahanukar et al., 2007)

Molecular data

A 9.7kb Gr64f promoter fragment is fused upstream of sequence encoding a GAL4 driver.

(Weiss et al., 2011)

Approximately 10kb of Gr64f promoter sequence is fused upstream of sequence encoding a GAL4 driver.

(Dahanukar et al., 2007)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

S cell | subset of prothoracic tarsal segment 2

(Ling et al., 2014)

S cell | subset of prothoracic tarsal segment 3

(Ling et al., 2014)

S cell | subset of prothoracic tarsal segment 5

(Ling et al., 2014)

S cell | subset of prothoracic tarsal segment 4

(Ling et al., 2014)

S cell | subset of mesothoracic tarsal segment 5

(Ling et al., 2014)

S cell | subset of mesothoracic tarsal segment 4

(Ling et al., 2014)

S cell | subset of metathoracic tarsal segment 5

(Ling et al., 2014)

S cell | subset of metathoracic tarsal segment 4

(Ling et al., 2014)

gustatory receptor neuron of labellum sensillum | subset

(Weiss et al., 2011)

S cell of labellum sensillum

(Weiss et al., 2011)

L-type sensillum L1 | intense

(Weiss et al., 2011)

L-type sensillum L2 | intense

(Weiss et al., 2011)

L-type sensillum L4 | intense

(Weiss et al., 2011)

L-type sensillum L6 | intense

(Weiss et al., 2011)

L-type sensillum L8 | intense

(Weiss et al., 2011)

L-type sensillum L9 | intense

(Weiss et al., 2011)

L-type sensillum L3 | intense

(Weiss et al., 2011)

L-type sensillum L5 | intense

(Weiss et al., 2011)

L-type sensillum L7 | intense

(Weiss et al., 2011)

S-type sensillum S0 | intense

(Weiss et al., 2011)

S-type sensillum S1 | intense

(Weiss et al., 2011)

S-type sensillum S2 | intense

(Weiss et al., 2011)

S-type sensillum S3 | intense

(Weiss et al., 2011)

S-type sensillum S4 | intense

(Weiss et al., 2011)

S-type sensillum S5 | intense

(Weiss et al., 2011)

S-type sensillum S6 | intense

(Weiss et al., 2011)

S-type sensillum S7 | intense

(Weiss et al., 2011)

S-type sensillum S8 | intense

(Weiss et al., 2011)

S-type sensillum S10 | intense

(Weiss et al., 2011)

S-type sensillum S11 | intense

(Weiss et al., 2011)

S-type sensillum S12 | intense

(Weiss et al., 2011)

I-type sensillum I1 | intense

(Weiss et al., 2011)

I-type sensillum I3 | intense

(Weiss et al., 2011)

I-type sensillum I4 | intense

(Weiss et al., 2011)

I-type sensillum I5 | intense

(Weiss et al., 2011)

I-type sensillum I6 | intense

(Weiss et al., 2011)

I-type sensillum I7 | intense

(Weiss et al., 2011)

I-type sensillum I8 | intense

(Weiss et al., 2011)

I-type sensillum I9 | intense

(Weiss et al., 2011)

I-type sensillum I10 | intense

(Weiss et al., 2011)

gustatory receptor neuron | subset of prothoracic tarsal segment 4

(Fan et al., 2013)

gustatory receptor neuron | subset of prothoracic tarsal segment 5

(Fan et al., 2013)

S cell of labellar taste bristle

(Fujii et al., 2015)

prothoracic leg taste bristle chemosensory neuron of tarsal segment 4

(Fujii et al., 2015)

S cell of prothoracic leg taste bristle chemosensory neuron

(Fujii et al., 2015)

prothoracic leg taste bristle chemosensory neuron of tarsal segment 5

(Fujii et al., 2015)

adult subesophageal zone | restricted

(Fujii et al., 2015)

S cell

(Thoma et al., 2016)

prothoracic leg taste bristle ascending tarsal chemosensory neuron

(Thoma et al., 2016)

prothoracic leg taste bristle segmental tarsal chemosensory neuron

(Thoma et al., 2016)

prothoracic leg taste bristle chemosensory neuron

(Ahn et al., 2017)

Additional Information

Statement

Reference

Drives expression in the sugar-responsive neuron of all of the short, intermediate and long taste sensilla of the labellum.

(FlyBase Curators, 2017)

Scer\GAL4^Gr64f.9.7 drives expression in sweet taste receptor neurons in the labellar taste hairs, and in about 12 sugar-sensing chemosensory neurons in the prothoracic leg. A subset of the leg neurons project to the ventral nerve cord then ascend through the cervical connective to the posterior gnathal gangion; these neurons, unlike the other Scer\GAL4^Gr64f.9.7-expression neurons, do not coexpress Scer\GAL4^Gr5a.8.5 or Ecol\lexA^{VP16.Gr5a.cGa}

(Thoma et al., 2016)

All labellar and many of the tarsal S cells expressing Scer\GAL4^Gr64f.9.7 send projections to the adult subesophageal zone.

(Fujii et al., 2015)

Scer\GAL4^Gr64f.9.7 is strongly expressed in the sugar-responsive neuron of all of the short, intermediate and long taste sensilla of the labellum.

(Weiss et al., 2011)

Scer\GAL4^Gr64f.9.7 is expressed in all the sugar-sensitive receptor neurons of the gustatory sensilla of the labellum.

(Weiss et al., 2011)

Marker for

S cell

(Ganguly et al., 2017, Fujii et al., 2015)

prothoracic leg taste bristle chemosensory neuron

(Ahn et al., 2017)

Reflects expression of

Gr64f

(Ahn et al., 2017, Thoma et al., 2016, Fujii et al., 2015, Ling et al., 2014, Fan et al., 2013, Weiss et al., 2011, Weiss et al., 2011)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Bloomington

57669

w^*; P{Gr64f-GAL4.9.7}5/CyO; MKRS/TM2

57668

w^*; P{UAS-mCD8::GFP.L}LL5; P{Gr64f-GAL4.9.7}1/TM3, Sb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gr64f

(Shankar et al., 2015)

Gr64f-Gal4

(Li and Montell, 2021, Li et al., 2020, Youn et al., 2018, Delventhal et al., 2017, Kim et al., 2017, Mohammad et al., 2017, Sánchez-Alcañiz et al., 2017, Talay et al., 2017, Jourjine et al., 2016, Wang et al., 2016, Yapici et al., 2016, Kwon et al., 2014, Sun et al., 2014, Thistle et al., 2012, Park and Kwon, 2011)

P{Gr64f-GAL4.9.7}

(FlyBase, 1992-)

Secondary FlyBase IDs

References (46)

Report Sections

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