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General Information
Symbol
P{ID.GAL4AD}
FlyBase ID
FBtp0064530
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Marker(s)
Description

Donor construct for the InSITE system, designed to swap the coding region present in an InSITE target enhancer trap insertion with sequence encoding a GAL4(AD)::Zip+ hemidriver. Contains an FRT cassette which contains an attB site, a minimal P\T promoter followed by the GAL4(AD)::Zip+ hemidriver sequence, and a w+mC marker (the marker is flanked by loxP sites). This donor cassette can be excised by FLP recombinase and then inserted into the attP site of an InSITE target insertion using phiC31:int integrase. The swap can then be completed by using P1cre recombinase to remove the original target GAL4 coding region (this step should also result in the removal of the w+mC marker).

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
selectable
marker
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (4)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
pXN-FBLWLF-GAL4AD
pXN-FBLWLF-Gal4AD
Secondary FlyBase IDs
    References (4)