Recombinant construct PBac{681.P.FSVS-1}

General Information
Symbol	PBac{681.P.FSVS-1}	FlyBase ID	FBtp0065989
Feature type	transgenic_transposon

Size	12.209	Expression data
Associated insertions	160 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role	GFP gene trap (FlyProt project., 2009.11)
Description	CV term Qualifiers & info Reference transposon P-element (FlyProt project., 2009.11) Tni\piggyBac (FlyProt project., 2009.11)
Uses	CV term Qualifiers & info Reference gene trap vital (FlyProt project., 2009.11) genomic insertion screen protein trap
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	12.209 (FlyProt project., 2009.11)
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (FlyProt project., 2009.11) molecular tag (FlyProt project., 2009.11) reporter gene (FlyProt project., 2009.11) selectable marker (FlyProt project., 2009.11)
Component Alleles
Allele	Avic\GFP^{Venus.FSVS-1.T:Zzzz\FLAG,T:Zzzz\StrepII}
Reference(s)	(FlyProt project., 2009.11)
Molecular data	Consists of the open reading frame of the "Venus" variant of Avic\GFP which is tagged at both the N-terminal and C-terminal end with T:Zzzz\StrepII. 3 copies of the T:Zzzz\FLAG tag are present at the N-terminal end of the open reading frame. A splice acceptor site from the Mhc gene is present at the 5' end of the open reading frame and a splice donor site from the Mhc gene is present at the 3' end of the open reading frame. Splice frame 1 variant. (FlyProt project., 2009.11)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(FlyProt project., 2009.11)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	PBac{602.P.SVS-1} (FlyProt project., 2009.11)
descendant(s)
Comments
	Hybrid piggyBac/P-element protein trapping vector containing 3xFLAG-StrepTag-Venus-StrepTag. (FlyProt project., 2009.11) Splice frame = 1 (comparable to splice frame in P{PTT-GA}.) (FlyProt project., 2009.11)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{681.P.FSVS-1} (FlyBase, 1996-) PIGP681{FSVS-1} (FlyProt project., 2009.11)
Secondary FlyBase IDs

References ( 4 )
Research paper	Hijazi et al., 2011, PLoS ONE 6(3): e17763 The ly6 protein coiled is required for septate junction and blood brain barrier organisation in Drosophila. [FBrf0213269] Rees et al., 2011, Mol. Cell. Proteomics 10(6): M110.002386 In Vivo Analysis of Proteomes and Interactomes Using Parallel Affinity Capture (iPAC) Coupled to Mass Spectrometry. [FBrf0213845]
Personal communication to FlyBase	FlyProt project., 2011.8.8, CPTI protein trap lines available from the Kyoto Stock Center. CPTI protein trap lines available from the Kyoto Stock Center. [FBrf0214588] FlyProt project., 2009.11, Notes on constructs used in generating CPTI protein trap lines. (Computer file) Notes on constructs used in generating CPTI protein trap lines. (Computer file) [FBrf0213656]