Recombinant construct P{5-HT7-GAL4.B}

General Information
Symbol	P{5-HT7-GAL4.B}	FlyBase ID	FBtp0068087
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Becnel et al., 2011)
Uses	CV term Qualifiers & info Reference binary system (regulatory) 5-HT7 (Becnel et al., 2011)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Becnel et al., 2011)
Component Alleles
Allele	Scer\GAL4^5-HT7.ERGP
Reference(s)	(Becnel et al., 2011, Johnson et al., 2011, Luo et al., 2012, Kahsai et al., 2012)
Molecular data	A 5' enhancer region of 5-HT7 corresponding to 5kb of genomic DNA immediately upstream of the ATG start codon is fused to Scer\GAL4 coding sequences in an 'Enhancer Ready Gal4-P element' vector. (Becnel et al., 2011)
Phenotypic class	courtship behavior defective, with 5-HT7^{dsRNA.Sym.Scer\UAS} (Becnel et al., 2011) mating behavior defective, with 5-HT7^{dsRNA.Sym.Scer\UAS} (Becnel et al., 2011)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage embryonic/larval brain \| restricted (Becnel et al., 2011) ventral nerve cord \| restricted (Becnel et al., 2011) adult stage adult brain \| restricted (Becnel et al., 2011) ring neuron (Becnel et al., 2011) bulb (Becnel et al., 2011, Kahsai et al., 2012) antennal lobe \| faint & restricted (Becnel et al., 2011) thoracico-abdominal ganglion (Becnel et al., 2011) ellipsoid body anterior shell (Kahsai et al., 2012) ellipsoid body posterior shell \| restricted (Kahsai et al., 2012) adult brain cortex \| restricted (Kahsai et al., 2012)
Additional Information
Statement Reference Scer\GAL4[5-HT7.ERGP] labels a serotonergic receptor and it is expressed in subsets of cells in the larval brain and ventral nerve cord. In the adult, labelling is mostly in the central complex, including 48 ring neurons of the ellipsoid body and the lateral triangles (bulbs). Co-localisation with Ddc is observed in the central complex. There is also weak staining in some antennal lobe glomeruli and in one neuron that innervates it. This neuron does not express Ddc. One cluster of cells labelled by Scer\GAL4[5-HT7.ERGP] in the lateral brain is in close proximity to a group of cells which express Pdf. Scer\GAL4[5-HT7.ERGP] also drives expression in paired neurons of all thoracic and abdominal neuromeres of the thoracico-abdominal ganglion: 40 and 30 neurons, respectively. In the larval brain and ventral nerve cord and in the adult thoracico-abdominal ganglion the absence of co-localisation between Scer\GAL4[5-HT7.ERGP] and Ddc suggests that Scer\GAL4[5-HT7.ERGP] is expressed postsynaptically. (Becnel et al., 2011) Scer\GAL4[5-HT7.ERGP] drives expression in the anterior and posterior rings of the ellipsoid body. In the latter, expression is observed in the innermost and outermost ring. Expression is also detected in approximately 30 paired cell bodies in the anterior cortex that project to the bulb (lateral triangle) and to the ellipsoid body. (Kahsai et al., 2012)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	5-HT₇Dro-GAL4 (Becnel et al., 2011) 5-HT₇-GAL4 (Kahsai et al., 2012) 5-HT₇-Gal4 (Luo et al., 2012) GAL4-5-HT₇Dro (Johnson et al., 2011) P{5-HT7-GAL4.B} (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 4 )
Research paper	Kahsai et al., 2012, Neuroscience 208: 11--26 Distribution of metabotropic receptors of serotonin, dopamine, GABA, glutamate, and short neuropeptide F in the central complex of Drosophila. [FBrf0217851] Luo et al., 2012, Cell. Molec. Life Sci. 69(3): 471--484 Insulin-producing cells in the brain of adult Drosophila are regulated by the serotonin 5-HT(1A) receptor. [FBrf0217219] Becnel et al., 2011, PLoS ONE 6(6): e20800 The Serotonin 5-HT(7)Dro Receptor Is Expressed in the Brain of Drosophila, and Is Essential for Normal Courtship and Mating. [FBrf0213968] Johnson et al., 2011, Neuroscience 192: 372--381 Serotonin receptor activity is necessary for olfactory learning and memory in Drosophila melanogaster. [FBrf0215034]