FlyBase Recombinant Construct Report: P{Ir76b-GAL4.916}

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Recombinant Construct: P{Ir76b-GAL4.916}

General Information

Symbol

P{Ir76b-GAL4.916}

FlyBase ID

FBtp0069672

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^Ir76b.916

Also Known As

IR76b-Gal4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^Ir76b.916

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Description

A 916bp fragment from immediately upstream of the Ir76b start codon is fused upstream of sequence encoding a GAL4 driver.

(Silbering et al., 2011)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^Ir76b.916

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

antennal coeloconic sensillum ac1

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac2

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac3

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac4

(Silbering et al., 2011)

antennal lobe glomerulus VM1

(Silbering et al., 2011)

antennal lobe glomerulus VM4

(Silbering et al., 2011)

antennal lobe glomerulus VC3

(Silbering et al., 2011)

antennal lobe glomerulus VM6

(Silbering et al., 2011)

leg taste bristle | subset

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic leg taste bristle chemosensory neuron

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic tarsal segment 2

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic tarsal segment 3

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic tarsal segment 4

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic tarsal segment 5

(Ganguly et al., 2017)

pharyngeal sense organ

(Ganguly et al., 2017)

labellar taste peg

(Ganguly et al., 2017)

gustatory receptor neuron | subset of labellar taste bristle

(Ganguly et al., 2017)

gustatory receptor neuron of adult abdomen

(Sánchez-Alcañiz et al., 2018)

gustatory receptor neuron of wing

(Sánchez-Alcañiz et al., 2018)

mesothoracic leg taste bristle chemosensory neuron | subset

(Sánchez-Alcañiz et al., 2018)

metathoracic leg taste bristle chemosensory neuron | subset

(Sánchez-Alcañiz et al., 2018)

prothoracic leg taste bristle chemosensory neuron | subset

(Sánchez-Alcañiz et al., 2018)

dorsal cibarial sense organ neuron

(Sánchez-Alcañiz et al., 2018)

ventral cibarial sense organ neuron

(Sánchez-Alcañiz et al., 2018)

labral sense organ neuron

(Sánchez-Alcañiz et al., 2018)

gustatory receptor neuron of labellar taste bristle

(Sánchez-Alcañiz et al., 2018)

gustatory receptor neuron of labellar taste peg

(Sánchez-Alcañiz et al., 2018)

adult antennal olfactory receptor neuron | subset

(Sánchez-Alcañiz et al., 2018)

neuron of labellum | restricted

(Hussain et al., 2016)

neuron of adult antennal lobe | restricted

(Hussain et al., 2016)

neuron of leg | restricted

(Hussain et al., 2016)

wing margin | restricted

(Hussain et al., 2016)

Additional Information

Statement

Reference

ScerGAL4^Ir76b.916 drives expression in ~24 S cells in metathoracic tarsal segment 5 and ~11 in metathoracic tarsal segment 3. The remaining metathoracic leg tarsal segments show sex specific differences, with tarsal segment 4 having ~14 neurons in females and ~22 in males, while tarsal segment 2 has ~10 in females and ~15 in males. Expression near completely overlaps with that of ScerGAL4^Ir76b.1.5, except that ScerGAL4^Ir76b.916 does not label L1 GRNs in the labellar taste bristles, some cells in the labellum nor axonal projections in the adult subesophageal zone that are labelled by the former.

(Ganguly et al., 2017)

ScerGAL4^Ir76b.916 is expressed in the neurons that innervate the antennal coeloconic sensillum ac1-a4 and in the antennal lobe glomeruli VM1, VM4, VC3 and VC5.

(Silbering et al., 2011)

Marker for

antennal lobe glomerulus VC3

(Silbering et al., 2011)

antennal lobe glomerulus VM1

(Silbering et al., 2011)

antennal lobe glomerulus VM4

(Silbering et al., 2011)

antennal lobe glomerulus VM6

(Silbering et al., 2011)

Reflects expression of

(Sánchez-Alcañiz et al., 2018, Ganguly et al., 2017, Hussain et al., 2016, Silbering et al., 2011)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

41730

w^*; P{Ir76b-GAL4.916}226.8; TM2/TM6B, Tb⁺

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

IR76b-GAL4

(Silbering et al., 2011)

IR76b-Gal4

(Toshima and Schleyer, 2024, Boehm et al., 2022, Croset et al., 2016, Hussain et al., 2016)

Ir76b-GAL4

(Task et al., 2022, Steck et al., 2018, Aso et al., 2014)

Ir76b-GAL4^RB

(Ganguly et al., 2017)

Ir76b-Gal4

(Münch et al., 2022, Sánchez-Alcañiz et al., 2018, Rist and Thum, 2017)

P[IR76b-GAL4.916]

(Hussain et al., 2016)

P{Ir76b-GAL4.916}

(FlyBase, 1992-)

Secondary FlyBase IDs

References (15)

Report Sections