Recombinant construct P{GMR-w.IR}

General Information
Symbol	P{GMR-w.IR}	FlyBase ID	FBtp0070260
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	4 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Lee and Carthew, 2003, Lee et al., 2004)
Uses	CV term Qualifiers & info Reference characterization w (Lee et al., 2004) GMR construct w (Lee et al., 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference ectopic expression (Lee et al., 2004)
Component Alleles
Allele	w^dsRNA.GMR
Reference(s)	(Harris et al., 2011, Lee and Carthew, 2003, Vagin et al., 2006, Ghildiyal et al., 2008, Kupsco et al., 2006, Lim et al., 2008, Marques et al., 2010, Schneiderman et al., 2010, Forstemann et al., 2005, Lee et al., 2004, van Rij et al., 2006, Kim et al., 2007, Lee et al., 2009, Zhou et al., 2008, Nayak et al., 2010, Pelisson et al., 2007, Fukunaga et al., 2012, Ji et al., 2012)
Molecular data	Construct: Consists of two copies of the w exon 3 in opposite orientation to each other and separated by a w intron. The construct is terminated with a Hsp70 terminator. The resulting transcript after intron splicing, is predicted to create a perfect hairpin dsRNA of length 629 bp. (Lee et al., 2004) Construct: The GMR promoter drives expression of a tail-to-tail w repeat. The repeat comprises the 629bp third exon of w ligated to the 74bp second intron of w, ligated to another copy of the w third exon in the opposite orientation to the first copy. (Lee and Carthew, 2003)
Phenotypic class	eye color defective (Lee and Carthew, 2003, Kupsco et al., 2006, Pelisson et al., 2007) visible (Lee and Carthew, 2003, Fukunaga et al., 2012)
Phenotype manifest in	eye (Kupsco et al., 2006, Pelisson et al., 2007, Fukunaga et al., 2012) pigment cell (Lee and Carthew, 2003)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 2 )
Bloomington	32068 P{GMR-w.IR}13D; P{neoFRT}40A r2d2^S165fsX 32067 P{GMR-w.IR}13D; P{neoFRT}82B Dcr-1^Q1948X/TM3, Sb¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	GMR-w^IR (Ji et al., 2012) GMR-wIR (Harris et al., 2011, Lim et al., 2008, Schneiderman et al., 2010, Lee et al., 2004, Kim et al., 2007, Lee et al., 2009, Zhou et al., 2008, Pelisson et al., 2007, Fukunaga et al., 2012) GMRwIR (Kupsco et al., 2006) IR^white (Nayak et al., 2010) IR-white (Forstemann et al., 2005) P[GMR-whiteIR] (Lee and Carthew, 2003) P{GMR-w.IR} (FlyBase, 1996-, ) P{GMR-wIR} (FlyBase, 1996-, van Rij et al., 2006)
Secondary FlyBase IDs
	FBtp0018219 FBtp0020657
References ( 19 )
Research paper	Fukunaga et al., 2012, Cell 151(3): 533--546 Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals. [FBrf0219804] Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Harris et al., 2011, J. Cell Biol. 194(1): 77--87 Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing. [FBrf0214287] Marques et al., 2010, Nat. Struct. Mol. Biol. 17(1): 24--30 Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila. [FBrf0209625] Nayak et al., 2010, Nat. Struct. Mol. Biol. 17(5): 547--554 Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. [FBrf0210742] Schneiderman et al., 2010, PLoS Genet. 6(9): e1001095 Perturbation Analysis of Heterochromatin-Mediated Gene Silencing and Somatic Inheritance. [FBrf0211786] Lee et al., 2009, Nat. Cell Biol. 11(9): 1150--1156 Silencing by small RNAs is linked to endosomal trafficking. [FBrf0208810] Ghildiyal et al., 2008, Science 320(5879): 1077--1081 Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells. [FBrf0205170] Lim et al., 2008, Biochem. Biophys. Res. Commun. 371(3): 525--530 Functional analysis of dicer-2 missense mutations in the siRNA pathway of Drosophila. [FBrf0205367] Zhou et al., 2008, Mol. Cell 32(4): 592--599 Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. [FBrf0206362] Kim et al., 2007, RNA 13(1): 22--29 Conversion of pre-RISC to holo-RISC by Ago2 during assembly of RNAi complexes. [FBrf0195398] Pelisson et al., 2007, J. Virol. 81(4): 1951--1960 A novel repeat-associated small interfering RNA-mediated silencing pathway downregulates complementary sense gypsy transcripts in somatic cells of the Drosophila ovary. [FBrf0195158] Kupsco et al., 2006, RNA 12(12): 2103--2117 Genetic and biochemical characterization of Drosophila Snipper: A promiscuous member of the metazoan 3 ' hExo/ERI-1 family of 3 ' to 5 ' exonucleases. [FBrf0191914] Vagin et al., 2006, Science 313(5785): 320--324 A distinct small RNA pathway silences selfish genetic elements in the germline. [FBrf0194520] van Rij et al., 2006, Genes Dev. 20(21): 2985--2995 The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. [FBrf0194879] Forstemann et al., 2005, PLoS Biol. 3(7): e236 Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein. [FBrf0188210] Lee et al., 2004, Cell 117(1): 69--81 Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. [FBrf0174449] Lee and Carthew, 2003, Methods 30(4): 322--329 Making a better RNAi vector for Drosophila: use of intron spacers. [FBrf0160727]
Supplementary material	Horiuchi et al., 2005, Curr. Biol. 15(23): Supplemental data. [FBrf0191753]