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General Information
Symbol
Mi{PT-GFSTF.1}
FlyBase ID
FBtp0072599
Feature type
transgenic_transposon
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon_modified_in_vivo
Construct components
Product class / Tool use(s)
Encoded product / tool
Description

Mi{PT-GFSTF.1} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with an intron phase 1 protein trap cassette from the pBS-KS-attB1-2-PT-SA-SD-1-EGFP-FlAsH-StrepII-TEV-3xFlag plasmid. The protein trap cassette is composed of a splice acceptor site, a (GGS)[[4]] flexible linker, the 'GFSTF' multiple tag cassette (EGFP-Tag:CALI(TC)-Tag:StrepII-Tag:CS(TEVp)-3xTag:FLAG), another (GGS)[[4]] flexible linker and a splice donor site.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (230)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Mi{PT-GFSTF.1}
Secondary FlyBase IDs
    References (12)