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General Information
Symbol
P{UAS-Oamb-Tango}
FlyBase ID
FBtp0072867
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele Arr1::TEV\NIaUAS.Tango
Product class / Tool use(s)
Description

UAS regulatory sequences drive expression of two coding regions separated by a 2A peptide sequence that are designed to be used to report neuromodulator release in vivo using the 'Tango' system (PMID:18165312). The upstream coding region encodes a tethered transcription factor which is composed of either the Dop1R1 or Oamb receptor (depending on the particular construct) fused via a Tag:CS(TEVp) cleavage site to a lexA::UnkAD driver (the driver is tagged with the Tag:HA epitope) (this coding region is represented in FlyBase by the Dop1R1UAS.Tango and OambUAS.Tango alleles respectively). The downstream coding region consists of Arr1 fused to the TEV\NIa protease (this coding region is represented in FlyBase by the Arr1::TEV\NIaUAS.Tango allele). Upon activation of the Dop1R1 or Oamb receptor by its ligand, the Arr1::TEV\NIa protein is recruited, resulting in cleavage at the Tag:CS(TEVp) site. This allows the Tag:HA-tagged lexA::UnkAD driver to translocate to the nucleus, where it can drive expression of a reporter that is controlled by lexAop regulatory sequences.

Component allele OambUAS.Tango
Product class / Tool use(s)
Description

UAS regulatory sequences drive expression of two coding regions separated by a 2A peptide sequence that are designed to be used to report neuromodulator release in vivo using the 'Tango' system (PMID:18165312). The upstream coding region encodes a tethered transcription factor which is composed of the Oamb receptor fused via a Tag:CS(TEVp) cleavage site to a lexA::UnkAD driver (the driver is tagged with the Tag:HA epitope) (this coding region is represented in FlyBase by the OambUAS.Tango allele). The downstream coding region consists of Arr1 fused to the TEV\NIa protease (this coding region is represented in FlyBase by the Arr1::TEV\NIaUAS.Tango allele). Upon activation of the Oamb receptor by its ligand, the Arr1::TEV\NIa protein is recruited, resulting in cleavage at the Tag:CS(TEVp) site. This allows the Tag:HA-tagged lexA::UnkAD driver to translocate to the nucleus, where it can drive expression of a reporter that is controlled by lexAop regulatory sequences.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
chimeric
protein
molecular
tag
Component Alleles
Reference(s)
Molecular data

UAS regulatory sequences drive expression of two coding regions separated by a 2A peptide sequence that are designed to be used to report neuromodulator release in vivo using the 'Tango' system (PMID:18165312). The upstream coding region encodes a tethered transcription factor which is composed of either the Dop1R1 or Oamb receptor (depending on the particular construct) fused via a Tag:CS(TEVp) cleavage site to a lexA::UnkAD driver (the driver is tagged with the Tag:HA epitope) (this coding region is represented in FlyBase by the Dop1R1UAS.Tango and OambUAS.Tango alleles respectively). The downstream coding region consists of Arr1 fused to the TEV\NIa protease (this coding region is represented in FlyBase by the Arr1::TEV\NIaUAS.Tango allele). Upon activation of the Dop1R1 or Oamb receptor by its ligand, the Arr1::TEV\NIa protein is recruited, resulting in cleavage at the Tag:CS(TEVp) site. This allows the Tag:HA-tagged lexA::UnkAD driver to translocate to the nucleus, where it can drive expression of a reporter that is controlled by lexAop regulatory sequences.

Reference(s)
Molecular data

UAS regulatory sequences drive expression of two coding regions separated by a 2A peptide sequence that are designed to be used to report neuromodulator release in vivo using the 'Tango' system (PMID:18165312). The upstream coding region encodes a tethered transcription factor which is composed of the Oamb receptor fused via a Tag:CS(TEVp) cleavage site to a lexA::UnkAD driver (the driver is tagged with the Tag:HA epitope) (this coding region is represented in FlyBase by the OambUAS.Tango allele). The downstream coding region consists of Arr1 fused to the TEV\NIa protease (this coding region is represented in FlyBase by the Arr1::TEV\NIaUAS.Tango allele). Upon activation of the Oamb receptor by its ligand, the Arr1::TEV\NIa protein is recruited, resulting in cleavage at the Tag:CS(TEVp) site. This allows the Tag:HA-tagged lexA::UnkAD driver to translocate to the nucleus, where it can drive expression of a reporter that is controlled by lexAop regulatory sequences.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

The P{UAS-Oamb-Tango} construct can be used as a reporter of octopamine release in vivo.

Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{UAS-Oamb-Tango}
UAS-DopR-Tango
Secondary FlyBase IDs
    References (3)