FB2025_01 , released February 20, 2025
Recombinant Construct: P{ppk23-Gal4.2.695}
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General Information
Symbol
P{ppk23-Gal4.2.695}
FlyBase ID
FBtp0079723
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4ppk23.2.695
Also Known As
ppk23-Gal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4ppk23.2.695
Transgenic product class
Component
Use(s)
Regulatory region(s)
ppk23
Encoded product / tool
GAL4
Description

A 2.695kb upstream fragment of ppk23 regulates expression of a GAL4 driver.

(Thistle et al., 2012)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
gustatory receptor neuron of terminal organ dorsolateral group
(Mast et al., 2014)
gustatory receptor neuron of terminal organ distal group | subset
(Mast et al., 2014)
adult stage
gustatory receptor neuron | subset of adult prothoracic segment
(Clowney et al., 2015)
neuron | restricted of labellum
(Shankar et al., 2015)
neuron | restricted of prothoracic leg
(Shankar et al., 2015)
gustatory receptor neuron of the labellum | subset
(Lee et al., 2018)
calcium-sensing neuron of labellar S-type taste bristle
(Lee et al., 2018)
labellar taste bristle chemosensory neuron | subset
(Thistle et al., 2012)
leg taste bristle chemosensory neuron | subset
(Thistle et al., 2012)
adult gnathal ganglion | restricted
(Thistle et al., 2012)
adult ventral nerve cord | restricted
(Thistle et al., 2012)
Additional Information
Statement
Reference

Drives expression in calcium-sensing neuron of labellar S-type taste bristles, and in a subset of leg taste bristle chemosensory neurons that project into the adult ventral nerve cord; and in gustatory receptor neurons of the terminal organ in larvae.

(FlyBase Curators, 2017)

Scer\GAL4ppk23.2.695 drives expression in 10 neurons that innervate the terminal organ, and in one internal sensory neuron located along the larval pharynx.

(Mast et al., 2014)

ScerGAL4ppk23.2.695 drives expression in gustatory receptor neurons in the proboscis and in the legs. These neurons labeled with ScerGAL4ppk23.2.695 do not overlap with expression of ScerGAL4ppk28.PC, which labels the water-sensing neurons, ScerGAL4Gr64f.9.7 which labels the sugar-responsive neurons,or with candidate pheromone receptor ScerGAL4Gr32a.3.776. A few positive ScerGAL4ppk23.2.695 neurons in the proboscis co-express AvicGFPGr66a.3x.IRES.2x which labels bitter-responsive neurons, but there is no co-expression in the leg sensory neurons. Expression of ScerGAL4ppk23.2.695 in the legs marks more than one cell per bristle and it overlaps with that of fruP1.LexA.

(Thistle et al., 2012)

There is sexual dimorphism in the leg gustatory receptor neurons labeled by ScerGAL4ppk23.2.695 : twice as many cells are labeled in males compared to females and the neuronal projections of males in the prothoracic ganglion cross the midline whereas female ones do not.

(Thistle et al., 2012)
 
Marker for
Reflects expression of
ppk23
(Clowney et al., 2015, Shankar et al., 2015, Thistle et al., 2012)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
93026
w1118; P{ppk23-Gal4.2.695}2; TM2/TM6B
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (29)