FB2025_01 , released February 20, 2025
Recombinant Construct: P{snRNA:U7-GFP}
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General Information
Symbol
P{snRNA:U7-GFP}
FlyBase ID
FBtp0082062
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPsnRNA:U7
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPsnRNA:U7
Transgenic product class
Component
Use(s)
Encoded product / tool
GFP
Description

The snRNA:U7 gene, including the putative 3' processing signals, are cloned upstream of GFP coding sequences, followed by a strong canonical polyA sequence, downstream of the stop codon. Constitutes a reporter for snRNA misprocessing; under normal conditions, the construct should produce a properly processed snRNA:U7 snRNA and GFP should not be expressed. However, if snRNA processing is impaired, since the snRNA:U7 sequences prior to the GFP ORF do not contain a AUG start codon, the resultant mRNA is expected to become polyadenylated, encode a functional GFP protein, and contain the snRNA:U7 sequence as its 5'UTR.

(Ezzeddine et al., 2011)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{snRNA:U7-GFP}
(FlyBase, 1992-)
U7-GFP
(Ezzeddine et al., 2011)
Secondary FlyBase IDs
    References (2)