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General Information
Symbol
PBac{I-SceI(FRT.RabX4-GAL4.ORF(loxP.3xP3-RFP))}
FlyBase ID
FBtp0083967
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
rabX4-Gal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Tni\piggyBac
Construct components
Component allele Scer\GAL4RabX4.ORF
Product class / Tool use(s)
Regulatory region(s)
Description

Contains a genomic fragment centred on RabX4 in which the complete RabX4 open reading frame has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
Component Alleles
Molecular data

Contains a genomic fragment centred on RabX4 in which the complete RabX4 open reading frame has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.

Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4RabX4.ORF drives expression in almost all neurons and possibly glia in the larval, pupal and adult brain. Expression is also detected in the developing photoreceptors in the larval eye disc and in the adult photoreceptor cells. Expression in the adult mushroom body is stronger than in the pupa.

 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

The PBac{I-SceI(FRT.RabX4-GAL4.ORF(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the RabX4 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous RabX4 locus.

Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PBac{I-SceI(FRT.RabX4-GAL4.ORF(loxP.3xP3-RFP))}
RabX4-Gal4
rabX4-Gal4
Secondary FlyBase IDs
    References (6)