FlyBase Recombinant Construct Report: P{UAS-Cas9.P}

A Drosophila codon-optimized Cas9 endonuclease fused to the Tag:NLS(Ude) nuclear localization signal is expressed under the control of UAS regulatory sequences (ORF cloned into P{10XUAS-IVS-Syn21-GFP-p10}, FBtp0073107).

(Port and Bullock, 2014.1.10)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Spyo\Cas9^{UAS.Tag:NLS(Ude)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Zzzz\Cas9 is derived from Streptococcus pyogenes.

(Port and Bullock, 2014.1.10)

Stocks (3)

Stocks containing insertion(s) of this recombinant construct

Bloomington

54594

P{hsFLP}1, y¹ w¹¹¹⁸; P{UAS-Cas9.P}attP40

54592

P{hsFLP}1, y¹ w¹¹¹⁸; P{UAS-Cas9.P}attP2/TM6B, Tb¹

54593

P{hsFLP}1, y¹ w¹¹¹⁸; P{UAS-Cas9.P}attP2 P{GAL4::VP16-nanos.UTR}CG6325^MVD1

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

P{UAS-Cas9.P}

(Port and Bullock, 2014.1.10, FlyBase, 1992-)

UAS-Cas9

(Bustillo et al., 2024, Zhang et al., 2024, Godneeva et al., 2023, Silva et al., 2023, Szabó et al., 2023, Kosakamoto et al., 2022, Llobet Rosell et al., 2022, Azpurua et al., 2021, Clay et al., 2021, Naidu et al., 2020, Trivedi et al., 2020, Hudry et al., 2019)

UAS-Cas9.P

(Ibaraki et al., 2021, Christensen et al., 2020, Huynh et al., 2018)

UAS-Cas9.P1

(Port et al., 2020)

UAS-cas9

(Port et al., 2015, Port et al., 2014)

UAS>Cas9

(Perry et al., 2021)

UASCas9

(Pathak et al., 2017)

UAScas9

(Ravi et al., 2018)

pUAS-cas9.P1

(Port and Bullock, 2016)

Secondary FlyBase IDs

References (25)

Report Sections

Open Close