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General Information
Symbol
P{UAS-tkv-TIPF}
FlyBase ID
FBtp0093190
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
UAS-TIPF, UAS-tkv-TIPF
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
Construct components
Component allele tkvTIPF.UAS
Product class / Tool use(s)
Description

UASt regulatory sequences drive expression of 'TIPF', a sensor that can be used to assay tkv activation. It consists of tkv isoform-D coding sequences fused to the conformation-sensitive circularly permutated yellow fluorescent protein (cpYFP) and FKBP12 (a motif that binds to the unphosphorylated glycine-serine rich domain of inactive type I BMP receptors). Activation of tkv displaces the FKBP12 motif, allowing cpYFP to adopt a fluorescent conformation.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
molecular
tag
Component Alleles
Molecular data

UASt regulatory sequences drive expression of 'TIPF', a sensor that can be used to assay tkv activation. It consists of tkv isoform-D coding sequences fused to the conformation-sensitive circularly permutated yellow fluorescent protein (cpYFP) and FKBP12 (a motif that binds to the unphosphorylated glycine-serine rich domain of inactive type I BMP receptors). Activation of tkv displaces the FKBP12 motif, allowing cpYFP to adopt a fluorescent conformation.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Secondary FlyBase IDs
    References (7)